萝卜硫素对心肌缺血再灌注损伤大鼠心肌保护作用及色氨酸-犬尿氨酸通路的影响  

Effects of sulforaphane on myocardial protection and tryptophan-kynurenine pathway in rats with myocardial ischemia reperfusion injury

作　　者：王帅 朱超男 邬鹏宇 崔志成 赵长全 WANG Shuai;ZHU Chaonan;WU Pengyu;CUI Zhicheng;ZHAO Changquan(Department of Cardiovascular Surgery,Affiliated Hospital of North China University of Science and Technology,Tangshan,Hebei 063000,China;Department of Thoracic Surgery,Affiliated Hospital of North China University of Science and Technology,Tangshan,Hebei 063000,China)

机构地区：[1]华北理工大学附属医院心脏大血管外科,河北唐山063000 [2]华北理工大学附属医院胸外科,河北唐山063000

出　　处：《安徽医药》2023年第1期60-64,I0005,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探究萝卜硫素(SFN)对心肌缺血再灌注(I/R)损伤大鼠色氨酸(TRP)-犬尿氨酸(KYN)信号通路及心肌损伤的影响。方法研究于2019年7月至2020年6月进行,将SD雄性大鼠随机分为假手术组(Control)、模型组(I/R)、SFN低(I/R+L SFN)、高(I/R+H SFN)剂量组。术后24 h,ELISA检测各组大鼠血清中炎性因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)含量;HE染色观察各组大鼠心肌组织病理变化;TUNEL检测各组大鼠缺血心肌细胞凋亡情况;HPLC检测各组大鼠心肌组织中TRP、KYN浓度;蛋白质印迹法(Western blotting)检测各组大鼠心肌组织B细胞淋巴瘤-2相关X蛋白(Bax)、B细胞淋巴瘤-2(Bcl-2)、活化胱天蛋白酶3(cleaved-capase3)、吲哚胺2,3-双加氧酶(IDO)蛋白表达情况。结果与Control组相比,I/R大鼠心肌细胞破碎、坏死,细胞排列不规则,心肌纤维断裂,伴有炎性细胞浸润,血清中炎性因子IL-1β、IL-6、TNF-α含量、心肌细胞凋亡指数[(32.85±3.13)比(1.07±0.08)]、心肌组织TRP[(783.75±30.32)pmol/L比(402.23±23.55)pmol/L]、KYN浓度[(567.54±28.18)pmol/L比(215.31±20.03)pmol/L]、KYN/TRP比值、cleaved-capase3、Bax、IDO蛋白水平显著升高(P<0.05),Bcl-2蛋白水平显著降低(P<0.05);与I/R相比,I/R+L SFN、I/R+H SFN剂量组心肌细胞上述病理症状明显减轻,大鼠血清中炎性因子IL-1β、IL-6、TNF-α含量、心肌细胞凋亡指数、心肌组织TRP、KYN浓度、KYN/TRP比值、Bax、cleaved-capase3、IDO蛋白水平依次降低(P<0.05),Bcl-2蛋白水平显著升高(P<0.05)。结论SFN可减少心肌细胞凋亡,缓解机体炎症反应,对心肌损伤有保护作用可能与TRP-KYN信号通路有关。Objective To explore the effect of sulforaphane(SFN)on the tryptophan(TRP)-kynurenine(KYN)signaling pathway and myocardial injury in rats with myocardial ischemia reperfusion(I/R)injury.Methods From July 2019 to June 2020,SD male rats were randomly assigned into sham operation group(Control),model group(I/R),SFN low group(I/R+L SFN),and high group(I/R+H SFN)dose groups.24h after operation,the serum levels of inflammatory factors interleukin 1β(IL-1β),interleukin 6(IL-6)and tumor necrosis factorα(TNF-α)in each group were detected by ELISA;HE staining was used to observe the pathological changes of rat myo‐cardial tissue;TUNEL was used to detect the apoptosis of ischemic myocardial cells in each group;HPLC was used to detect the con‐centration of TRP and KYN in rat myocardial tissue;western blot was used to detect the protein expression of B cell lymphoma-2 associ‐ated X protein(Bax),B cell lymphoma-2(Bcl-2),cleaved cysteine aspartate proteolytic enzyme 3(cleaved-capase3)and indoleamine 2,3-dioxygenase(IDO)in the myocardial tissue of each group.Results Compared with the control group,myocardial cells of I/R rats were broken and necrotic,the cell arrangement was irregular,myocardial fibers were broken,accompanied by inflammatory cell infiltra‐tion,and the serum levels of inflammatory factors IL-1β,IL-6 and TNF-α,myocardial cell apoptosis index[(32.85±3.13)vs.(1.07±0.08)],myocardial tissue TRP[(783.75±30.32)pmol/L vs.(402.23±23.55)pmol/L],KYN concentration[(567.54±28.18)pmol/L vs.(215.31±20.03)pmol/L],KYN/TRP ratio,cleaved-capase3,Bax,IDO protein levels were significantly increased(P<0.05),Bcl-2 protein levels were significantly reduced(P<0.05);Compared with I/R,I/R+L SFN and I/R+H SFN dose groups have significantly reduced the above-mentioned pathological symptoms of cardiomyocytes,the serum levels of inflammatory factors IL-1β,IL-6,and TNF-αcardio‐myocyte apoptosis index,myocardial tissue TRP,KYN concentration,KYN/TRP ratio,Bax,cleaved-capase3,IDO protein levels were decreased(P<0.05),but th

关 键 词：再灌注损伤 莱菔硫素 心肌缺血 色氨酸-犬尿氨酸信号通路 BCL-2相关X蛋白质 胱天蛋白酶3 白细胞介素类 肿瘤坏死因子α 

分 类 号：R285.5[医药卫生—中药学]