小麦XHS基因家族鉴定与表达分析  

Identification and Expression Analysis of XHS Gene Family in Wheat

作　　者：梁婷 王伟伟 汪德州 陆青 吴娴 王小燕[1] 唐益苗[2] Liang Ting;Wang Weiwei;Wang Dezhou;Lu Qin;Wu Xian;Wang Xiaoyan;Tang Yimiao(College of Agriculture,Yangtze University,Jingzhou,434025;Beijing Engineering and Research Institute for Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100097)

机构地区：[1]长江大学农学院,荆州434025 [2]北京市农林科学院杂交小麦研究所,北京100097

出　　处：《分子植物育种》2022年第20期6638-6655,共18页Molecular Plant Breeding

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20200423)和北京市农林科学院协同创新中心建设方案项目(KJCX201907-2)共同资助。

摘　　要：为了明确X1-homologous genes(XHS)基因家族在小麦基因组中的数量、结构及在干旱、高温处理下的表达差异,本研究对小麦XHS家族进行鉴定,并对编码蛋白理化性质、进化关系、顺式作用元件及其组织表达模式和干旱、高温处理下表达模式进行分析。研究表明,共鉴定得到69个TaXHSs基因家族成员,根据系统发育关系,可分为4个亚类。亚细胞定位预测表明,大部分TaXHSs蛋白定位在细胞核,少数定位在中心体、线粒体或细胞质。顺式作用元件主要与激素、生长发育和胁迫响应相关。组织表达数据显示,TaXHSs主要在穗和种子中表达。此外,TaXHSs的高温响应模式与干旱、高温双重胁迫下的响应模式相似。非生物胁迫下表达模式分析表明,TaXHS29-A、TaXHS9-B不受干旱和高温胁迫诱导表达,TaXHS23-D在高温处理下根部表达量显著上调;TaXHS30-B在干旱胁迫根部表达量显著升高,此外该基因的叶片组织受高温胁迫表达量也显著上调。本研究为改良小麦抗逆性提供理论依据。This work was main to identify the X1 homologous(XHS)gene members in common wheat and to analysis the expression patterns under drought and high temperature stress,which could be helpful to reveal the function of wheat XHS genes under stress conditions.The physical and chemical properties,evolutionary relationship,cis acting elements and tissue specific expressions,as well as the expression patterns under drought and high temperature treatment of wheat XHS family were analyzed.A total of 69 TaXHSs members were identified,which could be divided into four subclasses according to the phylogenetic analysis.Subcellular localization prediction showed that most TaXHSs proteins were located in the nucleus,and a few in the centrosome,mitochondria,or cytoplasm.Cis acting elements analysis suggested that most of this family members were related to hormones,growth and development and stress response.Tissue expression pattern showed that TaXHSs were mainly expressed in panicles and seeds.The expression model of TaXHSs under high temperature stress and drought and high temperature stress was almost the same.RT-qPCR analysis showed that TaXHS29-A and TaXHS9-B genes were not induced by drought and high temperature stress,and the expression level of TaXHS23-D gene was significantly up-regulated un der high temperature treatment.The expression level of TaXHS30-B gene in seedling without root tissues was significantly up-regulated under drought stress,meanwhile the expression in leaves was also significantly up-regulated under high temperature stress.This work could provide a theoretical basis to improve the stress resistance in common wheat.

关 键 词：小麦 XHS基因家族 逆境胁迫 表达模式 

分 类 号：S512.1[农业科学—作物学]