玉竹根茎转录组分析及其甾体皂苷生物合成途径探究  

Transcriptome Analysis of Polygonatum odoratum Rhizome and Study on Its Steroid Saponins Biosynthesis Pathway

作　　者：殷乐 佐月 黄心意 韩鹏 闫艳 许永华 邸鹏 Yin Le;Zuo Yue;Huang Xinyi;Han Peng;Yan Yan;Xu Yonghua;Di Peng(National Engineering Research Center of Ginseng Breeding and Application,College of Traditional Chinese Medicine,Jilin Agricultural University,Changchun,130118)

机构地区：[1]吉林农业大学中药材学院,人参新品种选育与开发国家地方联合工程研究中心,长春130118

出　　处：《分子植物育种》2022年第20期6705-6713,共9页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2017YFC1702101);吉林省重点科技研发项目(20180201006YY);国家自然科学基金项目(81703635)共同资助。

摘　　要：甾体皂苷是玉竹的主要有效成分,是玉竹降血糖、调节免疫力和消炎作用的物质基础。为解析玉竹中甾体皂苷生物合成途径,本研究通过分析玉竹根茎转录组数据,筛选出参与甾体皂苷骨架合成的82个Unigenes,通过RT-qPCR分析,验证了其中的17个Unigenes;通过系统进化树分析,鉴定出OSC基因家族中的环阿屯醇合酶和羊毛甾醇合酶。这些发现可用于玉竹甾体皂苷生物合成途径关键酶基因功能和调控机制的进一步研究,有助于黄精属其他植物甾体皂苷生物合成途径的研究。Steroid saponins are the main effective components of Polygonatum odoratum,and are the material basis of P.odoratum lowering blood glucose,regulating immunity and anti-inflammatory.In order to analyze the biosynthetic pathway of steroidal saponins in P.odoratum,this study analyzed the transcriptome data of P.odoratum rhizome and screened out 82 unigenes involved in the biosynthesis of steroidal saponins backbone,and verified 17 unigenes through RT-qPCR analysis;through phylogenetic tree analysis,cycloartenol synthase and lanosterol synthase in the OSC gene family were identified.These findings can be used to further study the functions and regulatory mechanisms of critical enzyme in the biosynthetic pathway of steroidal sapogenins in P.odoratum,and contribute to the research on the biosynthetic pathways of steroidal saponins in Polygonatum.

关 键 词：玉竹 转录组 甾体皂苷 生物合成途径 

分 类 号：S567.239[农业科学—中草药栽培]