毛尖紫萼藓GpPrxⅡ基因的克隆及表达分析  

Cloning and Expression Analysis of GpPrxⅡGene in Grimmia pilifera

作　　者：张梅娟[1] 敖佳 马天意 钱朋智[2] 王韬 沙伟[1] Zhang Meijuan;Ao Jia;Ma Tianyi;Qian Pengzhi;Wang Tao;Sha Wei(College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihar,161006;College of Food and Bioengineering,Qiqihar University,Qiqihar,161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006 [2]齐齐哈尔大学食品与生物工程学院,齐齐哈尔161006

出　　处：《分子植物育种》2022年第20期6714-6720,共7页Molecular Plant Breeding

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(135509319);齐齐哈尔市科技计划市校合作项目(SXSP-2020003);国家自然科学基金项目(31570207)共同资助。

摘　　要：以毛尖紫萼藓干旱cDNA文库中获得的一段与PrxⅡ基因同源性较高的EST序列为基础,采用RACE技术获得该基因cDNA全长序列,命名为GpPrxⅡ,并对其进行生物信息学分析和基因表达分析。结果显示,该cDNA全长805 bp,包含489 bp的开放阅读框,编码162个氨基酸蛋白质,含有中心模序PTCS。生物信息学分析结果显示,该蛋白质为稳定蛋白,分子质量为17.49 kD,理论等电点pI为6.07,不属于跨膜蛋白且不存在信号肽。进化分析显示,此基因编码蛋白与小立碗藓PrxⅡ蛋白亲缘关系最近。RT-qPCR结果显示,GpPrxⅡ在复水和快速干旱模式下均能表达,推测该基因可能参与毛尖紫萼藓的干旱和复水胁迫过程,为后续进一步研究其功能特征奠定了基础。A full-length cDNA of a novel peroxiredoxins gene named GpPrxⅡwas cloned from Grimmia pilifera through the method of rapid amplification of cDNA ends(RACE).The results showed that the full length of this gene was 805 bp,and contained a 489 bp open reading frame which encoded a protein containing 162 amino acids,containing the active site motif PTCS.The results of bioinformatics analysis showed that this protein was a stable protein,which molecular weight was 17.49 kD and theoretical pI was 6.07,and it was not a transmembrane protein and had no signal peptide.Phylogenetic analysis indicated that this gene had the closest genetic relationship with that of Physcomitrella patens.The real-time PCR results suggested that the expression of GpPrxⅡgene was induced in both rehydration and dehydration,this gene might play an important role in the two processes,and the results would lay the foundations for the further study on its function.

关 键 词：毛尖紫萼藓 GpPrxⅡ 基因克隆 生物信息学 表达分析 

分 类 号：Q943.2[生物学—植物学]