拟斯卑尔脱山羊草高分子量麦谷蛋白遗传多样性分析  

Genetic Diversity Analysis of High Molecular Weight Glutenins in Aegilops speltoides

作　　者：叶发慧 赵彩霞 李亚鑫 沈吉成 刘瑞娟[1,3] 刘德梅[1,3] 陈文杰[1,3] 张怀刚[1,2,3] Ye Fahui;Zhao Caixia;Li Yaxin;Shen Jicheng;Liu Ruijuan;Liu Demei;Chen Wenjie;Zhang Huaigang(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Innovation Academy for Seed Design,Chinese Academy of Sciencess,Xining,810008;University of Chinese Academy of Sciences,Beijing,100049;Qinghai Provincial Key Laboratory of Crop Molecular Breeding,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810008;College of Eco-Environmental Engineering,Qinghai University,Xining,810016)

机构地区：[1]中国科学院种子创新研究院,中国科学院西北高原生物研究所,中国科学院高原生物适应与进化重点实验室,西宁810008 [2]中国科学院大学,北京100049 [3]中国科学院西北高原生物研究所,青海省作物分子育种重点实验室,西宁810008 [4]青海大学生态环境工程学院,西宁810016

出　　处：《分子植物育种》2022年第20期6912-6917,共6页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2016YFD01-00500);中国科学院战略性先导科技专项(XDA2403-0102);青海省应用基础研究项目(2018-ZJ-701)共同资助。

摘　　要：为了揭示拟斯卑尔脱山羊草高分子量麦谷蛋白的遗传多样性情况,本研究通过对50份不同居群的拟斯卑尔脱山羊草材料进行了十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分析;首次使用基于相对迁移率的命名方法,对拟斯卑尔脱山羊草高分子量麦谷蛋白(HMW-GS)的谱带多态性进行了分析。结果表明,50份拟斯卑尔脱山羊草中,共分离出124条谱带,共含有43种不同的相对迁移率,其中10%的材料有小于相对迁移率1的谱带,未发现迁移率大于‘中国春’最后一条谱带的条带。材料间遗传相似系数(GS)的变幅为0~1,有两份材料的遗传相似系数为1,86.4%的GS值为0。本研究揭示了拟斯卑尔脱山羊草HMW-GS的遗传多样性,为源自拟斯卑尔脱山羊草新的HMW-GS亚基类型的发掘提供了依据。In order to reveal the genetic diversity of high molecular weight glutenin in Aegilops speltoides,this study was carried out by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)analysis of 50 different populations of the A.speltoides.In this study,the HMW-GS(high molecular weight glutenin subunits)of A.speltoides was analyzed with the nomenclature based on relative mobility for the first time.The results showed that 124 bans were isolated from 50 A.speltoides and contained 43 kinds of different relative mobilities.10%of the materials had bands smaller than the relative mobility 1,and none of the bands gad higher mobility than the last band of the’Chinese Spring’.The genetic similarity coefficient(GS)of two materials ranged from 0 to 1.There were two materials genetic coefficient was 1,and the GS value of 86.4%was 0.This study revealed the ge netic diversity of HMW-GS in Aegilops speltoid and provide the basis for the discovery of new HMW-GS subunits from A.speltoides。

关 键 词：拟斯卑尔脱山羊草 高分子量麦谷蛋白 十二烷基硫酸钠聚丙烯酰胺凝胶电泳 遗传多样性 

分 类 号：S512.1[农业科学—作物学]