大麦种质抗叶斑病鉴定和全基因组关联分析  

Identification of Spot Blotch Resistance in Barley(Hordeum vulgare)and Genome-wide Association Study

作　　者：曲洁琼 张毅 杨庆丽 汪军成[1,2] 姚立蓉 司二静[1,2] 马小乐[1,2] 李葆春[1,3] 方永丰[1,2] 王化俊[1,2] 孟亚雄[1,2] QU Jie-Qiong;ZHANG Yi;YANG Qing-Li;WANG Jun-Cheng;YAO Li-Rong;SI Er-Jing;MAXiao-Le;LI Bao-Chun;FANG Yong-Feng;WANG Hua-Jun;MENG Ya-Xiong(State Key Laboratory of Aridland Crop Science,Gansu Agricultural University/Gansu Provincial Key Lab of Crop Improvement&Germplasm Enhancement,Lanzhou 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;College of Life Sciences and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [2]甘肃农业大学农学院,兰州730070 [3]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《农业生物技术学报》2022年第12期2267-2278,共12页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31960426);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-05-04B-2);甘肃省教育厅优秀研究生创业之星项目(2021CXZX-401)。

摘　　要：大麦(Hordeum vulgare)是重要的作物和工业原料,大麦叶斑病(Spot blotch)是严重危害大麦生产的病害之一。该病害主要侵害叶片和叶鞘,其发生和流行严重影响大麦的产量和品质。为挖掘与大麦抗叶斑病相关联的分子标记,本研究通过在苗期人工接种叶斑病菌株对142份大麦材料进行抗性鉴定,并利用SSR分子标记结合大麦抗叶斑病表型鉴定结果进行关联分析,寻找有效抗叶斑病位点。结果表明,70个SSR标记共检测出504个等位变异,平均每个标记为7.2个;主基因频率变异范围是0.1963~0.5035,平均为0.3442;PIC变化范围为0.5212~0.8470;基因多样性指数的变化范围是0.6007~0.8624;聚类分析和群体遗传结构分析同时将该群体分为5类。结果共检测到6个显著关联的SSR标记,分别位于大麦的3H、4H和5H染色体上,并在4H染色体上最终确定了1个赋予大麦叶斑病抗性的QTL位点。本研究结果将有助于大麦品种的选育,获得的抗大麦叶斑病相关联位点可为大麦的分子标记辅助育种提供参考。Barley(Hordeum vulgare)is an important crop and industrial raw material,and spot blotch is one of the diseases that seriously endanger barley production.The disease mainly damages leaves and leaf sheaths,and its occurrence and prevalence seriously affect the yield and quality of barley.In order to explore the molecular markers related to barley spot blotch resistance,142 barley materials were identified by artificial inoculation of spot blotch pathogen at seedling stage,and SSR molecular markers combined with phenotypic identification results of barley resistance to spot blotch were used for correlation analysis,so as to find effective resistance sites.The results showed that 504 alleles were detected from 70 SSR markers,with an average of 7.2 alleles per marker.The variation range of major allele frequency was 0.1963 to 0.5035,with an average of 0.3442,and the variation range of PIC was 0.5212 to 0.8470.The genetic diversity index ranged from 0.6007 to 0.8624,and the population was divided into 5 groups by cluster analysis and population genetic structure analysis.A total of 6 SSR markers were detected,which were located on the 3H,4H and 5H chromosomes of barley,respectively,and a QTL locus that confered resistance to barley spot blotch was finally determined on the 4H chromosome.The results of this study will be helpful for the breeding of barley variety,and the associated loci of barley spot blotch resistance obtained can provide reference for molecular marker-assisted breeding of barley.

关 键 词：大麦 叶斑病抗性 遗传多样性 SSR 关联分析 

分 类 号：S4[农业科学—植物保护]