贝莱斯芽胞杆菌ZF145的诱变及抑菌防病效果研究  

Study on Mutagenesis,Bacteriostasis and Disease Prevention Effect of Bacillus velezensis ZF145

作　　者：刘世程 李磊 张雪艳[3] 石延霞[2] 柴阿丽[2] 范腾飞 李宝聚[2] 谢学文[2] LIU Shi-Cheng;LI Lei;ZHANG Xue-Yan;SHI Yan-Xia;CHAI A-Li;FAN Teng-Fei;LI Bao-Ju;XIE Xue-Wen(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;School of Agricultural,Ningxia University,Yinchuan 750021,China)

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]宁夏大学农学院,银川750021

出　　处：《农业生物技术学报》2022年第12期2417-2424,共8页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2020YFD1000400);国家大宗蔬菜产业技术体系(CRS-25);宁夏回族自治区重点研发计划(2021BBF03002);农业部园艺作物生物学与种质创制重点实验室项目(IVF2022)。

摘　　要：黄瓜细菌性角斑病是保护地黄瓜(Cucumis sativus)栽培中常见的细菌性病害之一,使用生防菌是一种重要的防治手段。为提高贝莱斯芽胞杆菌(Bacillus velezensis)对黄瓜细菌性角斑病的拮抗活性,分别采用紫外线(ultraviolet,UV)和室温常压等离子体(atmospheric room temperature plasma,ARTP)诱变的方式,对贝莱斯芽胞杆菌ZF145菌株进行诱变处理。以扁桃假单胞流泪致病变种(Pseudomonas amygdali pv.lachrymans)为指示菌,通过平板对峙和活体盆栽的方式对突变菌株进行筛选,并比较2种诱变方式突变效率,对正突变菌株进行遗传稳定性测试,分析正突变菌株代谢产物变化。结果表明,正突变菌株A561在活体盆栽实验中对黄瓜细菌性角斑病的防治效果高于出发菌株ZF145和对照药剂5%中生菌素可湿性粉剂,防治效果可达到63.61%,且生防相关代谢产物表面活性素合成基因簇(surfactin synthetase gene clusters,srf)基因srfAA、srfAB、srfAC表达量均显著上调(P<0.05)。ARTP诱变的正突变率为10.86%,远高于紫外线诱变正突变率,更适于芽胞杆菌的诱变选育。本研究为贝莱斯芽胞杆菌的诱变育种提供了理论支撑,对黄瓜细菌性角斑病生物防治具有重要意义。Bacterial angular spot of cucumber is one of the common bacterial diseases in the greenhouse cucumber(Cucumis sativus)cultivation.Using biocontrol bacteria is an important method to control the disease.In order to improve the antagonistic activity of Bacillus velezensis against bacterial angular spot of cucumber,the methods of ultraviolet(UV)mutagenesis and the atmospheric room temperature plasma(ARTP)mutagenesis were used to mutagenize the Bacillus velezensis strain ZF145.Taking Pseudomonas amygdali pv.Lachrymans as the indicator bacteria,screening was carried out by plate confrontation and in vivo experiment,and the mutation efficiency of the 2 mutagenesis methods was compared.The results showed that the control effect of positive mutant strain A561 on cucumber bacterial angular spot was 63.61%,which was much higher than that of ZF145 and the 5%Zhongshengmycin Wettable Powder.The expressions of the surfactin synthetase gene clusters(srf)genes srfAA,srfAB and srfAC associated with the synthesis of surfactin were significantly up-regulated(P<0.05).The positive mutation rate of ARTP mutation was 10.86%,much higher than that of UV mutation,which was more suitable for mutation breeding of Bacillus.This study provides a theoretical support for the mutation breeding of Bacillus velezensis,and is of great significance for the biological control of bacterial angular spot of cucumber.

关 键 词：黄瓜细菌性角斑病 贝莱斯芽胞杆菌 紫外线(UV) 室温常压等离子体(ARTP) 物理诱变 

分 类 号：S435.79[农业科学—农业昆虫与害虫防治]