酵母双杂交及其衍生技术应用研究进展  

Research Progress on Application of Yeast Two Hybrid System and Y2Hderivated Techniques

作　　者：沈竹 曹勤红[1] SHEN Zhu;CAO Qin-Hong(College of Biological Sciences,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学生物学院,北京100193

出　　处：《农业生物技术学报》2022年第12期2425-2433,共9页Journal of Agricultural Biotechnology

基　　金：中国农业大学生命科学实验教学示范中心探索项目(20210104)。

摘　　要：酵母双杂交(yeast two-hybrid,Y2H)系统是研究蛋白质相互作用的有效方法之一,Y2H系统在蛋白质组学、功能基因组学、病理学等研究领域发挥重要作用。由于Y2H的有效性,近年在其基础上发展出了许多衍生技术。本文介绍了Y2H、酵母单杂交、酵母三杂交、反向双杂交技术、分裂泛素化细胞质酵母双杂交系统等技术的原理,及其在高通量筛选、蛋白互作定量分析、蛋白质互作组学研究、致病机理的揭示、新药靶点的搜寻、农业等方面的应用。本文也展望了Y2H技术未来的发展方向。本综述有助于研究人员全面了解酵母双杂交技术的原理和发展历程,了解酵母双杂交技术的应用范畴,为需要使用该技术研究蛋白质互作的人员提供借鉴。The yeast two-hybrid(Y2H)system is one of the most effective molecular biology methods to identify protein-protein interactions.Y2H system has played an important role in proteomics,functional genomics,pathology and other research fields.Due to the success of Y2H,many derivative technologies based onY2H have been developed in recent years.The principle of Y2H,one-hybrid system,three-hybrid system,reverse two-hybrid system,cytosolic yeast two-hybrid system and other derivative systems were reviewed in this paper.The application of Y2H in high-throughput screening,quantitative analysis of protein interaction,protein interactome,pathogenic mechanism,search for new drug targets,and agriculture were introduced as well.The prospect of Y2H was also brought out in this article.This review will help researchers comprehend the principles and development process of Y2H.It can also help researchers understand the application scope of Y2H.This review would provide references for those who need to use Y2H to study protein-protein interactions.

关 键 词：酵母双杂交 蛋白质相互作用 蛋白质互作组学 酵母双杂交衍生技术 

分 类 号：S-1[农业科学] Q-31[生物学]