响应面法优化板蓝根中(R,S)-告依春和核苷类化合物的提取工艺  

Optimization of extraction process of (R,S)-epigoitrin and nucleoside from Radix isatidis by response surface methodology

作　　者：崔晓 薛淑一 孟向尚 李欠 CUI Xiao;XUE Shu-yi;MENG Xiang-shang;LI Qian(Department of Pharmacy,Qingdao Central Hospital,Qingdao 266042;College of Agronomy,Gansu Agricultural University,Lanzhou 730070)

机构地区：[1]青岛市中心医院药学部,青岛266042 [2]甘肃农业大学农学院,兰州730070

出　　处：《中南药学》2022年第11期2553-2558,共6页Central South Pharmacy

基　　金：国家自然科学基金项目(No.31860102)。

摘　　要：目的 优选板蓝根中(R,S)-告依春和核苷类化合物的提取工艺。方法 以尿苷、腺嘌呤、(R,S)-告依春、鸟苷、腺苷提取率为评价指标,采用多指标综合评分法,以液料比、超声功率、超声时间、酶反应时间、酶反应温度为影响因素,采用Box-Behnken响应面法优化板蓝根中(R,S)-告依春和核苷类化合物的提取工艺。结果 最佳提取条件为:液料比75∶1,超声功率400 W,超声时间50 min,酶反应时间8 h 40 min,酶反应温度50℃。在此条件下提取综合评分为97.137。结论 优化后的提取工艺操作简便,具有较高的推广价值。Objective To optimize the extraction process of (R,S)-epigoitrin and nucleoside from Radix isatidis.Methods The extraction rates of uridine,adenine,(R,S)-epigoitrin,guanosine and adenosine were used as the evaluation index.The multi-index comprehensive scoring method was used to determine the liquid-solid ratio,ultrasonic power,ultrasonic time,enzyme reaction time,and enzyme reaction temperature (the influencing factors).The Box-Behnken response surface methodology was used to optimize the extraction process of (R,S)-epigoitrin and nucleoside from Radix isatidis.Results The optimal extraction condition included:liquid-solid ratio at 75∶1,ultrasonic power 400 W,ultrasonic time 50 min,enzyme reaction time 8 h 40 min,and enzyme reaction temperature 50℃.The multi-index score was 97.137.Conclusion The optimized extraction process is simple with high promotion value.

关 键 词：板蓝根 (R S)-告依春 核苷 响应面法 黑芥子酶 提取工艺 

分 类 号：R284.2[医药卫生—中药学]