UPLC-Q-TOF-MS法分析椿乳凝胶中的化学成分  

Chemical constituents in Chunru gel by UPLC-Q-TOF-MS

作　　者：张逢雨 彭静[1,2] 王雨馨 蒋跃平[1,2] 姚亮元 袁红波 刘韶[1,2] ZHANG Feng-yu;PENG Jing;WANG Yu-xin;JIANG Yue-ping;YAO Liang-yuan;YUAN Hong-bo;LIU Shao(Department of Pharmacy,Xiangya Hospital of Central South University,Changsha 410000;National Clinical Research Center for Geriatric Diseases,Xiangya Hospital of Central South University-Laboratory of Rational and Safe Drug Use for Geriatrics Diseases,Changsha 410000;Zhuzhou Qianjin Pharmaceutical Co.,Ltd,Zhuzhou Hunan 412000)

机构地区：[1]中南大学湘雅医院药学部,长沙410000 [2]中南大学湘雅医院国家老年疾病临床医学研究中心-老年合理用药与安全用药研究室,长沙410000 [3]株洲千金药业股份有限公司,湖南株洲412000

出　　处：《中南药学》2022年第11期2580-2586,共7页Central South Pharmacy

基　　金：湖南省自然科学基金项目(No.2020JJ6100)。

摘　　要：目的 基于UPLC-Q-TOF-MS建立椿乳凝胶化学成分快速分析方法。方法 采用Agilent C clipsePlus C_(18)(2.1 mm×100 mm,1.8 μm);流动相为0.1%甲酸水-乙腈,梯度洗脱;流速0.30 mL·min^(-1)。正负离子采集模式下进行全扫描质谱。通过MassBank、NIST等数据库和Qualitative Workflows B.08.00,并结合相关文献检索以及质谱裂解规律分析椿乳凝胶中的化学成分,使用GC-MS法分析制剂中的挥发性成分。结果 共鉴定出106个化合物,其中57个化合物来自椿皮(2个与苦参共有,2个与牡丹皮共有,2个与乳香共有),44个化合物来自苦参(2个与椿皮共有),4个化合物来自牡丹皮(2个与椿皮共有),5个化合物来自乳香(2个与牡丹皮共有),2个化合物来自冰片。结论 本研究快速、准确地阐明了椿乳凝胶的活性成分,为进一步研究椿乳凝胶及其临床合理应用提供参考。Objective To establish a method for the rapid analysis of chemical constituents in Chunru gel by UPLC-Q-TOF-MS.Methods Agilent C clipsePlus C_(18)(2.1 mm×100 mm,1.8 μm) was used to identify the chemical constituents in Chunru gel.The mobile phase was 0.1% formic acid water-acetonitrile with gradient elution at 0.30 mL·min^(-1).Full scan mass spectrometry was performed in positive and negative ion acquisition mode.With MassBank,NIST and other databases and Qualitative Workflows B.08.00 combined with relevant literature search and mass spectrometry fragmentation rules,the chemical components in Chunru gel were analyzed.GC-MS technology was used to analyze the volatile components in the preparation.Results Totally 106 compounds were identified by UPLC-Q-TOF-MS.Among them,57 compounds were from Ailanthus altissima Swingle (2 shared with Sophora flavescens,2 shared with Chinese moutan cortex,and 2 shared with Frankincense);44 compounds were from Sophora flavescens (2 shared with Ailanthus altissima Swingle);4 compounds were from Chinese moutan cortex (2 shared with Ailanthus altissima Swingle);5 compounds were from Frankincense (2 shared with Ailanthus altissima Swingle);and 2 compounds were from borneol.Conclusion This method can quickly and accurately elucidate the active components in Chunru gel,and provide a reference for its further research and its rational clinical application.

关 键 词：椿乳凝胶 超高效液相色谱-质谱 化学成分 

分 类 号：R284.1[医药卫生—中药学]