Comparative chloroplast genomes of Ulva prolifera and U.linza(Ulvophyceae)provide genetic resources for the development of interspecific markers  

作　　者：Wenzheng LIU Qianchun LIU Jin ZHAO Xiu WEI Peng JIANG 

机构地区：[1]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]College of Life Science,Qingdao University,Qingdao 266071,China

出　　处：《Journal of Oceanology and Limnology》2022年第6期2372-2384,共13页海洋湖沼学报（英文）

基　　金：Supported by the Science&Technology Basic Resources Investigation Program of China(No.2018FY100205);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050302);the National Key R&D Program of China(No.2018YFD0901500);the National Natural Science Foundation of China(No.41776153);the Key R&D Program of Shandong Province(No.2019GSF107012)。

摘　　要：The green seaweeds Ulva linza and U.prolifera are closely related species.They usually co-occur widely and have important ecological significance as primary producers thriving in the intertidal zone.In the Yellow Sea,a genetically unique floating ecotype of U.prolifera even bloomed to cause serious green tides.However,there is still a lack of appropriate molecular markers to distinguish these two species,partially due to limited evaluations on the intraspecific variations in U.prolifera among dif ferent ecotypes.Since organelle genomes could provide rich genetic resources for phylogenetic analysis and development of genetic markers,in this study,the chloroplast genome from one attached population of U.prolifera was completely sequenced,and comparative genomic analyses were performed with other existing chloroplast genomes from U.linza and the floating ecotype of U.prolifera.The results showed that in spite of the high level of collinearity among three genomes,there were plenty of genetic variations especially within the non-coding regions,including introns and gene spacer regions.A strategy was proposed that only those signals of variation,which were identical between two ecotypes of U.prolifera but divergent between U.linza and U.prolifera,were selected to develop the interspecific markers for U.linza and U.prolifera.Two candidate markers,psa B and pet B,were shown to be able to distinguish these two closely related species and were applicable to more attached populations of U.prolifera from a wide range of geographical sources.In addition to the interspecific marker,this study would also provide resources for the development of intraspecific markers for U.prolifera.These markers might contribute to the surveys for Ulva species composition and green tide monitoring especially in the Yellow Sea region.

关 键 词：chloroplast genome comparative genomics intraspecifi c variation Ulva linza Ulva prolifera 

分 类 号：Q949[生物学—植物学]