Midkine ameliorates LPS-induced apoptosis of airway smooth muscle cells via the Notch2 pathway  

作　　者：Qi-Feng Huang Bo Wang Yu-Qing Weng Tang Deng Li-Hua Li Jin Qian Qi Li Kai-Wen Lin Dong-Mei Sun Shuang-Qin Xu Hang-Fei Wang Xin-Xin Wu Yuan-Tian Sun Xiao-Ran Liu 

机构地区：[1]Emergency Department,The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China [2]Jiangdong Health Center,Haikou 571126,China [3]Department of Pulmonary and Critical Care Medicines,Zhuhai People’s Hospital(Zhuhai Hospital Affiliated with Jinan University),Zhuhai 519050,China [4]College of Pharmacy,Hainan Medical University,Haikou 570100,China [5]Shandong Heze Mudan People’s Hospital,Heze 274007,China [6]Emergency and Trauma College,Hainan Medical University,Haikou 570100,China [7]College of Basic Medicine and Life Sciences,Hainan Medical University,Haikou 570100,China [8]Key Laboratory of Emergency and Trauma of Ministry of Education,Haikou 570100,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第12期512-519,共8页亚太热带生物医学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(NO.81660011,81960351);Hainan Provincial Social Development Foundation(NO.ZDYFXGFY2020004);Hainan Provincial Medical and Health Research Project(NO.22A200036).

摘　　要：Objective:To evaluate the effect of midkine on lipopolysaccharide(LPS)-induced airway smooth muscle cells(ASMCs).Methods:LPS-stimulated acute lung injury model was used to analyze the effect of midkine on ASMCs in vitro.Recombinant midkine and midkine siRNA were used to investigate the role of Notch2 signaling pathway.Cell proliferation was assessed using Cell Counting Kit-8 assay.Additionally,apoptosis was measured by flow cytometry and protein and mRNA expression of midkine and Notch2 was assessed by Western blotting and qPCR,respectively.Immunofluorescence analysis was also conducted.Results:LPS increased the mRNA and protein expression of midkine and Notch2.Midkine silencing reduced LPS-induced midkine and Notch2 expression.In addition,midkine silencing further reduced the viability and increased apoptosis of ASMCs induced by LPS,which was attenuated by recombinant midkine.Conclusions:The midkine/Notch2 signaling pathway plays a regulatory role in ASMC proliferation and apoptosis in airway inflammation.

关 键 词：Acute lung injury Airway smooth muscle cells MIDKINE NOTCH2 LIPOPOLYSACCHARIDE 

分 类 号：R563.8[医药卫生—呼吸系统]