甘参复方抗肝纤维化作用机制的代谢组学研究  

Metabolomic study on the mechanism of anti-hepatic fibrosis of glycyrrhiza uralensis-salvia miltrorrhiza prescription

作　　者：侯玉娇 张杰[1] 赵超阳 徐天娇[1] 李美谦 洪博[1] 李文静[1] HOU Yujiao;ZHANG Jie;ZHAO Chaoyang;XU Tianjiao;LI Meiqian;HONG Bo;LI Wenjing(School of Pharmacy,Qiqihar Medical College,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《中国比较医学杂志》2022年第11期68-74,112,共8页Chinese Journal of Comparative Medicine

基　　金：齐齐哈尔医学科学院青年博士专项科研基金项目(QMSI2020B-03)。

摘　　要：目的 该研究旨在从内源代谢物的角度阐明甘参复方(Glycyrrhiza Uralensis and Salvia Miltrorrhiza,GUSM)抗肝纤维化的作用机制。方法 皮下注射CCl溶液建立肝纤维化大鼠模型。通过肝形态学指标和HE染色评价GUSM的疗效。利用超高效液相色谱-飞行时间质谱技术,通过主成分分析、正交偏最小二乘判别分析法对正常组、模型组、GUSM组和阳性对照组大鼠的代谢物谱进行分析、筛选和鉴定。结果 共筛选和鉴定出16个与肝纤维化相关的潜在生物标志物。其中,牛磺胆酸、亚油酸、花生四烯酸等对肝纤维化进程影响较大。结论 甘参复方主要通过调节亚油酸和花生四烯酸的代谢,发挥抗HF作用。本研究有助于从代谢组学角度了解和分析GUSM抗HF的作用机制。Objective The purpose of this study is to comprehensively elucidate the anti-hepatic fibrosis mechanism of(Glycyrrhiza Uralensis and Salvia Miltrorrhiza,GUSM) from the perspective of endogenous metabolites. Methods Hepatic fibrosis rat model was established by subcutaneously injecting CClsolution. The efficacy of GUSM was evaluated by liver morphological indexes and HE method. We analyzed, screened and identified the metabolic profiles of rats from Normal group, Model group, GUSM group and Positive group by PCA and OPLS-DA method using UPLC-TOF-MS. Results A total of 16 potential biomarkers in both positive and negative mode associated with hepatic fibrosis were screened and identified. The metabonomics analysis revealed that Taurocholic acidf Glycocholic acid, Arachidonic acid, and so on were greatly disturbed. Conclusions GUSM prescription showed anti fibrosis effect on rats through regulating metabolic pathways, mainly about Arachidonic acid metabolism. This study is useful to better understand and analyze the anti hepatic fibrosis mechanism of GUSM prescription on HF rats using metabonomics.

关 键 词：甘参复方剂 代谢组学 超高效液相色谱-飞行时间质谱 肝纤维化 

分 类 号：R-33[医药卫生]