基因转录介导同质园条件下拟南芥不同地理种群的光合能力分化  

Photosynthetic Capacity Differentiation and Gene Transcription in Different Geographical Populations of Arabidopsis thaliana under Common Garden conditions

作　　者：李梦烁 刘莹泽 鲁焕 强胜[1] LI Mengshuo;LIU Yingze;LU Huan;QIANG Sheng(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095)

机构地区：[1]南京农业大学生命科学学院,南京210095

出　　处：《植物研究》2023年第1期90-99,共10页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(32102238)。

摘　　要：不同环境条件决定着植物光合能力的多态性,但在相同环境条件下同种植物不同种群间表现出光合能力分化的内在机制仍不清楚,本文旨在揭示同质园条件下欧洲拟南芥(Arabidopsis thaliana)不同地理种群光合能力的分化以及其基因转录调控机制。在同质园条件下,测定来自欧洲不同气候区的23个拟南芥的地理种群的气体交换参数、叶绿素荧光参数及SPAD值综合比较其光合能力差异。另外,根据测定结果选取光合能力差异的典型种群,利用实时定量PCR技术对光合相关基因表达水平进行验证。比较研究发现欧洲不同气候区拟南芥的地理种群间的气体交换参数差异较大,其中净光合速率的变化范围为2~11μmol·m^(-2)·s^(-1);而叶绿素荧光参数变异幅度较小,变异幅度几乎不超过10%。聚类分析表明23个拟南芥种群被分为强光合能力和弱光合能力2组,强光合种群主要分布在中欧和西欧地区,净光合速率平均为7.37μmol·m^(-2)·s^(-1);而弱光合种群则主要分布在东欧和南欧,净光合速率平均为4.46μmol·m^(-2)·s^(-1)。相关性分析发现净光合速率和水分利用效率及SPAD存在显著相关性。冗余分析(RDA)结果表明拟南芥光合能力的分化可能与其所在地区生长季温度与降雨量等环境因素有关。实时定量PCR试验表明,强光合能力的典型种群En-D和Stw-0的PSⅡ和Rubisco相关基因表达水平显著高于弱光合能力的典型种群Wa-1和Per-1,暗示着PSⅡ和Rubisco相关基因的转录差异可能参与了拟南芥种群光合能力分化的调控。本研究明确了同质园中不同拟南芥地理种群的光合能力存在差异,这种差异可能与原产地环境有关,并在长期进化中遗传给了后代。而PSⅡ和Rubisco相关基因转录调控可能参与了拟南芥光合能力的分化。Photosynthesis is a basic metabolism process for plants and provides the material basis for plant growth and development.Different environmental conditions determine the polymorphism of plant photosynthetic capacity,but the mechanisms regulating the divergence of photosynthetic capacity among different populations under the same environmental conditions remain still unknown.This study aims to reveal the divergence of photosynthetic capacity in different geographical populations of Arabidopsis thaliana in Europe under common garden conditions and the mechanisms of their gene transcriptional regulation.A comparative study of photosynthetic characteristics of 23 geographic A.thaliana(Arabidopsis)populations from different regions was conducted under common garden conditions by determining the gas exchange parameters,chlorophyll fluorescence parameters and SPAD values.In addition,the photosynthetic-related gene expressions of the typical populations with differences in photosynthetic capacity was examined using real-time quantitative PCR(RT-qPCR).The comparative result revealed that gas exchange parameters differed significantly among geographical populations of Arabidopsis from different climatic zones in Europe.The variation range of net photosynthetic rate was 2-11μmol·m^(-2)·s^(-1).while chlorophyll fluorescence parameters varied to a lesser extent,the variation range was almost no more than 10%.Cluster analysis showed that the 23 Arabidopsis populations were divided into two groups,strong and weak photosynthetic ability respectively.The populations with strong photosynthetic capacity were mainly distributed in central and western Europe,and the average net photosynthetic rate was 7.37μmol·m^(-2)·s^(-1).The populations with weak photosynthetic capacity were mainly distributed in eastern and southern Europe with an average net photosynthetic rate of 4.46μmol·m^(-2)·s^(-1).Correlation analysis revealed a significant correlation between net photosynthetic rate,water use efficiency and SPAD.The results of re

关 键 词：光合能力 同质园 PSⅡ基因 RUBISCO基因 基因表达 

分 类 号：Q945.3[生物学—植物学]