超声观察矽肺大鼠肺部声像图特征的实验研究  

Ultrasonic observation of lung sonographic features in silicosis rat:a experimental study

作　　者：杨金平[1] 朱林平 张灶玲[1] 陈瑞泽 汤永中 赵夏姿 YANG Jinping;ZHU Linping;ZHANG Zaoling;CHEN Ruize;TANG Yongzhong;ZHAO Xiazi(Department of Ultrasound Diagnosis,Guangxi Institute of Occupational Disease Prevention and Control,Nanning 530021,China)

机构地区：[1]广西壮族自治区职业病防治研究院超声诊断科,南宁市530021 [2]防城港京族医药研究院 [3]广西壮族自治区职业病防治研究院职业卫生与毒性评价所,南宁市530021 [4]广西壮族自治区职业病防治研究院放射科,南宁市530021

出　　处：《临床超声医学杂志》2022年第12期881-884,共4页Journal of Clinical Ultrasound in Medicine

基　　金：广西医疗卫生适宜技术开发与推广应用项目(S2021029);广西壮族自治区卫生健康委员会自筹经费科研课题(Z20200940);防城港市科学研究与技术开发计划项目(防科AB21014044)。

摘　　要：目的探讨超声观察矽肺大鼠肺部声像图特征的价值。方法选取SD大鼠17只,采用非暴露染尘法向大鼠气管内注入1 ml无菌矽尘混悬液,建立矽肺大鼠模型。所有大鼠均于染尘前和染尘后1个月行肺部超声检查,记录其肺部超声表现(胸膜线、B线、肺周围组织情况)。于染尘后1个月处死大鼠,取肺组织制作病理标本,光镜下观察大鼠肺组织病理表现。结果染尘前,所有大鼠肺部超声图像均表现为胸膜线连续性好、光滑、规则,无B线增多,无肺周围组织病变。染尘后1个月,9只大鼠肺周围组织见团状低回声,14只大鼠B线增多,17只大鼠胸膜线厚度与染尘前比较差异有统计学意义[(0.190±0.015)mm vs.(0.410±0.071)mm,P<0.05],14只大鼠胸膜线模糊、不规则且连续性中断。病理结果显示,染尘后1个月大鼠肺组织正常结构破坏,肺泡间隔增厚,细胞性矽结节形成,纤维组织增生,胶原纤维沉积增多。结论超声可观察大鼠染尘前后肺部声像图特征的变化,为临床诊断矽肺提供影像学依据。Objective To investigate the value of ultrasound in the observation of lung sonographic features in silicosis rat.Methods Seventeen SD rats were selected,and 1 ml sterile silica dust suspension was injected into the trachea of the rats by non-exposure dust contamination method to establish silicosis rat model.All rats were examined by lung ultrasound before and 1 months after dust exposure,ultrasound findings of lung were recorded(pleural line,B lines,surrounding lung tissue).The rats were sacrificed 1 month after the dust staining,and the lung tissues were taken to make pathological specimens.The pathological manifestations of the lung tissues of the rats were observed under the light microscope.Results Before dust staining,the lung ultrasound images of all rats showed that the pleural line was continuous,smooth and regular,there was no increase of B-line and no lesion in the peripulmonary tissue.1 month after the dust staining,the pulmonary ultrasound images showed hypoechoic mass in the surrounding lung tissues of 9 rats,B-line increased in 14 rats,difference in the thickness of pleural lines in 17 rats[(0.190±0.015)mm vs.(0.410±0.071)mm,P<0.05],blurred and irregular pleural lines and interrupted continuity in 14 rats. The pathological results showed that the normal structure of the lung tissue was destroyed,the alveolar septum was thickened,the cellular silicon nodule was formed,the fibrous tissue was hyperplasia,and the collagen fiber deposition was increased.Conclusion Ultrasound can observe the changes of lung sonographic features before and after dust staining,and can provide imaging reference for the clinical diagnosis of silicosis.

关 键 词：超声检查 肺 矽肺 大鼠 

分 类 号：R445.1[医药卫生—影像医学与核医学]