晚发型重度子痫前期胎盘组织LncRNA HIF1A-AS1、HIF1A表达与胎盘螺旋动脉重铸的关系  

The relationship between the expression of LncRNA HIF1A-AS1 and HIF1A in placental tissues with the recasting of placental spiral artery in late onset severe preeclampsia

作　　者：张静[1,3] 徐琳 叶元华[1,2] ZHANG Jing;XU Lin;YE Yuanhua(Qingdao University School of Medicine,Qingdao 266000,Shandong)

机构地区：[1]青岛大学医学院临床医学院,山东266000 [2]青岛大学医学院附属医院产科 [3]潍坊市人民医院产科,山东261000

出　　处：《中国生育健康杂志》2023年第1期51-55,共5页Chinese Journal of Reproductive Health

基　　金：国家自然科学基金(81571408)。

摘　　要：目的分析晚发型重度子痫前期孕妇胎盘组织长链非编码RNA缺氧诱导因子-1α-反义链1(LncRNA HIF1A-AS1)、缺氧诱导因子-1α(HIF1A)表达异常与胎盘螺旋动脉重铸的相关性。方法选取2019年10月—2021年4月于本院住院分娩的92例晚发型重度子痫前期孕妇为子痫前期组,选取同期86例正常妊娠孕妇作为正常妊娠组。收集各孕妇年龄、收缩压、舒张压等一般资料,采用实时荧光定量PCR(qRT-PCR)法检测胎盘组织LncRNA HIF1A-AS1、HIF1A mRNA水平,扫描电子显微镜下测定胎盘螺旋动脉管腔面积和管壁厚度;采用Pearson法分析晚发型重度子痫前期孕妇胎盘组织LncRNA HIF1A-AS1、HIF1A mRNA水平与各参数的相关性。结果与正常妊娠组相比,子痫前期组胎盘组织HIF1A mRNA水平及平均管壁厚度、收缩压、舒张压明显升高,LncRNA HIF1A-AS1水平、平均管腔面积明显降低,差异有统计学意义(P<0.05)。晚发型重度子痫前期孕妇胎盘组织LncRNA HIF1A-AS1与HIF1A mRNA水平呈负相关(r=-0.628,P<0.05),且LncRNA HIF1A-AS1与平均管腔面积呈正相关(P<0.05),与平均管壁厚度、收缩压、舒张压均呈负相关(P<0.05);HIF1A mRNA与平均管腔面积呈负相关(P<0.05),与平均管壁厚度、收缩压、舒张压均呈正相关(P<0.05)。结论晚发型重度子痫前期孕妇胎盘组织LncRNA HIF1A-AS1、HIF1A异常表达,可能与胎盘螺旋动脉重铸不足有关。Objective To analyze the relationship between the abnormal expression of long non-coding RNA hypoxia-inducible factor 1 alpha-antisense RNA 1(LncRNA HIF1A-AS1)and hypoxia-inducible factor-1 alpha(HIF1A)in placental tissues with the placental spiral artery recasting of pregnant women with late onset severe preeclampsia.Methods We selected ninety-two pregnant women with late onset severe preeclampsia who gave birth in our hospital from October 2019 to April 2021 as the preeclampsia group,and 86 pregnant women with normal pregnancy during the same period as the normal pregnancy group.We also collected the general data about age,systolic blood pressure and diastolic blood pressure of each pregnant woman.Real-time fluorescent quantitative PCR(qRT-PCR)method was used to detect the levels of LncRNA HIF1A-AS1 and HIF1A mRNA in the placental tissue,and scanning electron microscope was used to measure the lumen area and wall thickness of the placental spiral artery.We used Pearson′s method to analyze the correlation between LncRNA HIF1A-AS1 and HIF1A mRNA levels with different parameters in the placental tissues of pregnant women with late onset severe preeclampsia.Results Compared with the normal pregnancy group,the level of HIF1A mRNA in the placental tissue,average wall thickness,systolic blood pressure and diastolic blood pressure were significantly increased in the preeclampsia group.The LncRNA HIF1A-AS1 level and the average lumen area were significantly decreased(P<0.05).There was a negative correlation between LncRNA HIF1A-AS1 and HIF1A mRNA levels in the placental tissue of pregnant women with late onset severe preeclampsia(r=-0.628,P<0.05).LncRNA HIF1A-AS1 was positively correlated with the average lumen area(P<0.05),and was negatively correlated with the average tube wall thickness,systolic blood pressure and diastolic blood pressure(P<0.05).HIF1A mRNA level was negatively correlated with the average lumen area(P<0.05),and positively correlated with the average tube wall thickness,systolic blood pressure and d

关 键 词：晚发型重度子痫前期 胎盘组织 长链非编码RNA缺氧诱导因子-1α-反义链1 缺氧诱导因子-1Α 胎盘螺旋动脉重铸 

分 类 号：R71[医药卫生—妇产科学]