土家药三颗针对溃疡性结肠炎小鼠肠道菌群的影响  

Effect of Tujia medicine Berberidis Radix on intestinal flora in mice with ulcerative colitis

作　　者：胡雪黎 田力 李鸿 涂星 聂娟 杨宝 刘可云 胡泽华 HU Xue-li;TIAN Li;LI Hong;TU Xing;NIE Juan;YANG Bao;LIU Ke-yun;HU Ze-hua(Hubei Key Laboratory of Biologic Resources Protection and Utilization,Hubei Minzu University;Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases,Hubei Minzu University;Medical School,Hubei Minzu University;Chinese Medicinal Materials Products Quality Supevision and Inspection Center in Wuling Mountainous Area,Hubei Minzu University,Enshi 445000,China)

机构地区：[1]湖北民族大学生物资源保护与利用湖北省重点实验室 [2]湖北民族大学风湿性疾病发生与干预湖北省重点实验室 [3]湖北民族大学医学部 [4]湖北民族大学武陵山中药材检验检测中心,恩施445000

出　　处：《天然产物研究与开发》2022年第12期2069-2076,共8页Natural Product Research and Development

基　　金：国家自然科学基金(81560703);生物资源保护与利用湖北省重点实验室基金(PT012103);风湿性疾病发生与干预湖北省重点实验室基金(PT022210)。

摘　　要：本研究旨在探讨土家药三颗针对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导的溃疡性结肠炎(ulcerative colitis,UC)小鼠肠道菌群的影响,为其开发利用提供科学依据。60只c57BL/6J小鼠随机分为空白组、模型组、美沙拉嗪组以及三颗针低、中、高剂量组,自由饮用7 d 2.5%的葡聚糖硫酸钠溶液构建小鼠UC模型,同时给予药物干预,连续10 d,记录小鼠体重变化、疾病活动指数(disease activity index,DAI)。采用苏木精-伊红染色观察小鼠结肠病理变化,基于16S rRNA基因测序技术分析三颗针对UC小鼠肠道菌群的组成和多样性的影响。结果显示,与模型组相比,三颗针组DAI评分显著降低(P<0.05),结肠病理损伤减轻,肠黏膜结构较完整,炎性细胞浸润减少。三颗针改善了溃疡性结肠炎小鼠肠道菌群结构和多样性,且趋近于正常组。本实验揭示了调节肠道菌群多样性及菌群结构可能是三颗针干预UC的重要途径之一,通过肠道菌群的角度为三颗针的临床用药提供理论依据。This study aims to investigate Tujia medicine Berberidis Radix on intestinal flora of mice with ulcerative colitis induced by dextran sodium sulfate and provide the scientific basis for its development and utilization.c57BL/6J mice were randomly divided into normal group,model group,mesalazine group,and low-,medium-,and high-dose Berberidis Radix groups.Except for the normal group,2.5%dextran sodium sulfate drinking water was given to each group for seven days.At the same time,the administration group was gavaged with the mesalazine group and Berberidis Radix group for ten days.Body weight changes and disease activity index were measured.Hematoxylin-eosin staining was used to observe the pathological changes in colon tissue.The composition and diversity of the intestinal flora were detected by 16S rRNA gene sequencing.The results demonstrated that Berberidis Radix could significantly decrease disease activity index score(P<0.05).Berberidis Radix improved species richness and diversity in the intestinal flora of mice with ulcerative colitis and adjusted the abundance of the dysregulated bacteria closer to that of the normal group.This study reveals that regulating the diversity of intestinal flora and the structure of flora may be one of the essential ways for Berberidis Radix to intervene in ulcerative colitis and provides a theoretical basis for the clinical use of Berberidis Radix through the perspective of intestinal flora.

关 键 词：土家药 三颗针 肠道菌群 溃疡性结肠炎 16S rRNA基因测序 

分 类 号：R285.5[医药卫生—中药学]