LEF1基因在巴什拜羊不同毛色皮肤组织中的DNA甲基化及mRNA表达水平分析  

Analysis of DNA Methylation and mRNA Expression Level of LEF1 Gene in Skin Tissues of Bashbay Sheep with Different Wool Colors

作　　者：侯晨曦 洪文娟 何宗龙 决肯·阿尼瓦什[1] HOU Chenxi;HONG Wenjuan;HE Zonglong;Jueken Aniwashi(College of Animal Sciences,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830000

出　　处：《新疆农业科学》2022年第11期2742-2748,共7页Xinjiang Agricultural Sciences

基　　金：国家科技支撑(2015BAD29B06)。

摘　　要：【目的】分析LEF1基因在红棕色与青灰色巴什拜羊皮肤组织中DNA甲基化与mRNA的表达水平。【方法】运用BSP(亚硫酸盐修饰后测序PCR)与RT-RCR(实时荧光定量PCR),检测不同毛色巴什拜羊皮肤组织LEF1基因启动子区的甲基化水平与mRNA表达量。【结果】在红棕色巴什拜羊皮肤组织中的LEF1基因启动子区的甲基化水平高于青灰色的甲基化水平,且二者甲基化CpG位点不同,二者呈显著的负相关(P<0.05)。【结论】DNA甲基化水平对红棕色与青灰色巴什拜羊的毛色形成具有调节作用,可作为一个候选的巴什拜羊毛色遗传标记。【Objective】The purpose of this study is to analyze the DNA methylation and mRNA expression levels of LEF1 gene in skin tissues of red brown and blue gray Bashbay sheep.【Methods】The methylation level and mRNA expression of LEF1 gene promoter region in different skin tissues of Bashbay sheep were detected by BSP(sulfite Modified Sequencing PCR)and RT-RCR(real-time fluorescent quantitative PCR).【Results】The methylation level of LEF1 gene promoter in the skin tissue of red brown Bashbay sheep was higher than that of blue gray,and the methylation CpG_(s)ites of LEF1 gene promoter were different.At the same time,SPSS software was used to analyze the correlation between the DNA methylation level of LEF1 gene promoter and the mRNA expression,and it was found that there was a significant negative correlation between them(P<0.05).【Conclusion】The results show that DNA methylation level can regulate the formation of wool color in red brown and blue gray Bashbay sheep,and they can be used as a candidate genetic marker of Bashbay sheep wool color.

关 键 词：巴什拜羊 毛色 LEF1基因 MRNA表达量 DNA甲基化 

分 类 号：S826.8[农业科学—畜牧学]