五色培元固本方高效液相色谱指纹图谱及药效学研究  

HPLC Fingerprints and Pharmacodynamics of Wuse Peiyuan Guben Formula

作　　者：段瑶 陈娟[1] 沈洁 DUAN Yao;CHEN Juan;SHEN Jie(Department of Pharmacy,Chongqing Hospital of Traditional Chinese Medicine,Chongqing,China 400021)

机构地区：[1]重庆市中医院药剂科,重庆400021

出　　处：《中国药业》2022年第24期39-43,共5页China Pharmaceuticals

基　　金：重庆市自然科学基金面上项目[cstc2021jcjy-msxmX0886];重庆市科研机构绩效激励引导专项项目[jxyn2020-10];成都中医药大学“杏林学者”医院专项[YYZX2021062]。

摘　　要：目的 研究五色培元固本方的高效液相色谱(HPLC)指纹图谱及药物效应动力学。方法 采用HPLC法建立12批样品的HPLC图谱,利用中药色谱指纹图谱相似度评价系统(2012A版)进行相似度评价,确定共有峰。取20只SD大鼠,随机分为假手术组(6只,等体积蒸馏水)、模型组(7只,等体积蒸馏水)、五色培元固本方组(7只,9 g/kg);先切除大鼠2/3左肾,1周后切除右肾,然后连续8周喂饲4%酪蛋白饲料(5 g/100 g)以复制慢性肾脏病-蛋白质能量消耗(CKD-PEW)模型,假手术组仅作切口、剥离肾膜并喂饲常规饲料;建模同时灌胃相应药物或蒸馏水,每日1次,连续8周。称定大鼠体质量;采用酶联免疫吸附(ELISA)法检测大鼠血清肌酐(SCr)、血尿素氮(BUN)水平;检测24 h尿蛋白(Upr)水平;苏木素–伊红(HE)染色,观察大鼠肾脏、胫骨前肌病理组织形态变化;计算胫骨前肌横截面积。结果 12批样品的HPLC图谱有10个共有峰,相似度均大于0.95。与模型组比较,五色培元固本方组大鼠毛发光泽度较好,体质量增长速度较快,大小便频率较小,笼内洁净度较好,肾小管炎症减轻,细胞碎片及间质水肿减少;且BUN,SCr,24 h Upr水平均明显降低,胫骨前肌横截面积明显增大(P <0.05)。结论 五色培元固本方能有效改善CKD模型大鼠的蛋白质能量消耗。所建方法操作便捷,结果准确,重复性好,可用于五色培元固本方的质量控制和药效评价。Objective To study high-performance liquid chromatography(HPLC) fingerprints and pharmacokinetics of Wuse Peiyuan Guben Formula.Methods HPLC method was adopted to establish the HPLC chromatograms of 12 batches of samples,and the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2012A) was used to evaluate the similarity and determine the common peaks. Twenty SD rats were randomly divided into the sham operation group(six rats,equal volume of distilled water),operation group(seven rats,equal volume of distilled water) and Wuse Peiyuan Guben Formula group(seven rats,9 g/kg). Two-thirds of the left kidney of rats were resected first,then the right kidney was resected one week later,and then the rats were fed with 4% casein(5 g/100 g) for eight weeks to replicate the chronic kidney disease-protein energy wasting(CKD-PEW) model. However,the rats in the sham operation group were incised and their renal membrane was stripped,and fed with conventional diet. At the same time of modeling,the rats were administered with corresponding drugs or distilled water by gastric gavage once a day for eight consecutive weeks. The body mass of rats was weighed. The levels of serum creatinine(SCR) and blood urea nitrogen(BUN) were measured by the enzyme-linked immunosorbent assay(ELISA). The level of 24 h urinary protein(Upr) was detected. Hematoxylin-eosin(HE) staining was used to observe the pathological changes of kidney and tibialis anterior muscle. The cross-sectional area of tibialis anterior muscle was calculated.Results There were 10common peaks in the HPLC chromatograms of 12 batches of samples with similarity > 0. 95. Compared with those in the model group,the luster hair of rats was better,the growth rate of body weight was faster,the frequency of urination and defecation was less,the cleanliness of cage was better,the inflammation of renal tubule alleviated,the cell fragments and interstitial edema reduced,and the levels of BUN,SCr and 24 h Upr were significantly lower,and t

关 键 词：五色培元固本方 慢性肾脏病 大鼠 蛋白质能量消耗 药物效应动力学 

分 类 号：R932[医药卫生—生药学] R285[医药卫生—药学]