携载姜黄素纳米粒和新吲哚菁绿的透明质酸微针能抑制人舌鳞癌细胞增殖  

Hyaluronic acid microneedles loaded with curcumin nanodrugs and new indocyanine green inhibits human tongue squamous carcinoma cells in vitro

作　　者：谢稀 单越 张旭[1] 吴勇志 廖金凤 XIE Xi;SHAN Yue;ZHANG Xu;WU Yongzhi;LIAO Jinfeng(State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;Department of Orthodontics,Stomatology Hospital,School of Stomatology,Zhejiang University School of Medicine&Clinical Research Center for Oral Diseases of Zhejiang Province,Key Laboratory of Oral Biomedical Research of Zhejiang Province,Cancer Center of Zhejiang University,Hangzhou 310006,China)

机构地区：[1]四川大学华西口腔医院国家口腔疾病临床医学研究中心,口腔疾病研究国家重点实验室,四川成都610041 [2]浙江大学医学院附属口腔医院正畸科,浙江大学口腔医学院浙江省口腔疾病临床医学研究中心浙江省口腔生物医学重点实验室,浙江大学癌症研究院,浙江杭州310006

出　　处：《浙江大学学报（医学版）》2022年第5期585-593,共9页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(31972925)。

摘　　要：目的:制备并研究携载药物姜黄素纳米粒和光热触发剂新吲哚菁绿(IR820)的透明质酸微针(以下简称微针)给药系统对人舌鳞癌细胞(Cal-27)的抑制作用。方法:通过模板法分别制备对照微针、姜黄素纳米粒微针、IR820微针、姜黄素纳米粒+IR820微针,并分别进行形貌表征、力学强度测试、体外皮肤刺入试验及体外光热效应测试。将各组微针与Cal-27细胞共培养,其中IR820微针组及姜黄素纳米粒+IR820微针组用808 nm近红外光1 W/cm^(2)照射5 min,并通过活死细胞染色结果对各组细胞存活率进行统计。结果:制备的微针针体形貌均一,具有良好的力学强度及皮肤刺入能力,其中搭载IR820的微针具有较好的光热性能。与各组微针共培养后,Cal-27细胞存活率分别为空白对照组100.00%、对照微针组99.92%、姜黄素纳米粒微针组94.08%、IR820微针组0.41%、姜黄素纳米粒+IR820微针组0.04%。其中,姜黄素纳米粒+IR820微针组对肿瘤细胞杀伤效果最好,IR820微针组次之(均P<0.05)。结论:相比单一的药物治疗,姜黄素纳米粒+IR820微针对Cal-27细胞增殖具有更好的抑制作用。Objective:To prepare the hyaluronic acid microneedle(abbreviated as microneedle)delivery system carrying curcumin nanodrugs(Cur-NDs)and photothermal trigger agent new indocyanine green(IR820),and to investigate its effect on proliferation of human tongue squamous carcinoma cells(Cal-27)in vitro.Methods:The microneedle delivery system carrying Cur-NDs and IR820 was prepared.The morphological characteristics of the microneedles were observed,and the mechanical strength test,skin insertion ability test and the photothermal test in vitro were performed.Cal-27 cells were treated with microneedles,Cur-NDs microneedles,IR820 microneedles,or Cur-NDs+IR820 microneedles in vitro,respectively.The IR820 microneedle group and Cur-NDs+IR820 microneedle group were irradiated with 808 nm near infrared light at 1 W/cm^(2) for 5 min.The cell viability was tested with cell counting kit-8 method.Results:The prepared microneedles had homogeneous needle-like morphology,good mechanical strength and skin piercing ability,among which the microneedles equipped with IR820 showed better photothermal performance.The survival rates of Cal-27 cells were 100.00%in blank control group,99.92%in control microneedles group,94.08%in Cur-NDs microneedles group,0.41%in IR820 microneedles group,and 0.04%in Cur-NDs+IR820 microneedles group,respectively(all P<0.05).Conclusion:Compared with single drug treatment,Cur-NDs+IR820 microneedle shows better inhibitory effect on Cal-27 cell proliferation in vitro.

关 键 词：口腔鳞状细胞癌 微针 姜黄素纳米粒 光热治疗 人舌鳞癌细胞 

分 类 号：R318.08[医药卫生—生物医学工程]