土壤复合菌系产纤维素酶的影响因子优化  

Optimization of Factors Affecting Cellulase Production by Soil Composite Microbial System

作　　者：张敬敏[1] 桑茂鹏 赵智鹏 杨文霞[1] 王欣英[1] ZHANG Jingmin;SANG Maopeng;ZHAO Zhipeng;YANG Wenxia;WANG Xinying(Weifang University of Science and Technology,Shouguang Shandong 262700;Jingzhi-Maoteng Agricultural Technology Limited Company in Qushui County,Qushui Tibet 850600)

机构地区：[1]潍坊科技学院,山东寿光262700 [2]曲水县净植茂藤农业科技有限公司,西藏曲水850600

出　　处：《现代农业科技》2022年第24期129-132,共4页Modern Agricultural Science and Technology

基　　金：山东省重点研发计划(公益类)项目(2019GSF109113);2020年西藏自治区自然科学基金项目(XZ202001ZR0067G)。

摘　　要：以2种秸秆分解能力强的土壤复合菌系为试材,研究氮源、pH值和温度对复合菌系产纤维素酶的影响。结果表明:单因子试验中,LC和DT复合菌系均以氮源为尿素时,纤维素酶活性最高;LC复合菌在pH值为6、温度20℃时纤维素酶活性最高;DT复合菌系在pH值为6和温度为50℃时,纤维素酶活性最高。影响因子优化试验表明,LC复合菌系在氮源为尿素、pH值为6、温度为30℃时,纤维素酶活性最高,为148.60 U/L;DT复合菌系在氮源为尿素,pH值为7、温度为50℃时,纤维素酶活性最高,为141.32 U/L。Taking two soil composite microbial system with strong straw decomposition ability as the test material,this paper studied the effects of nitrogen source,pH value and temperature on cellulase production.The results showed that in the single factor experiment,when both LC and DT composite microbial system used urea as nitrogen source,the cellulase activity was the highest.LC composite microbial system had the highest cellulase activity when pH value was 6 and temperature was 20℃;DT composite microbial system had the highest cellulase activity when pH value was 6 and temperature was 50℃.The optimization test of influencing factors showed,when the nitrogen source was urea,the pH value was 6 and the temperature was 30℃,the cellulase activity of LC was the highest(148.60 U/L);when the nitrogen source was urea,pH value was 7 and temperature was 50℃,the cellulase activity of DT was the highest(141.32 U/L).

关 键 词：土壤 复合菌系 纤维素酶 影响因素 

分 类 号：S621.4[农业科学—园艺学]