Immunomodulatory effect of ethanol-soluble polypeptides from Atlantic cod (Gadus morhua)  

作　　者：Zhen Yuan Meilian Yang Dongyang Zhu Di Wu Shuzhen Cheng Chao Wu Hesham R.El-Seedi Ming Du 

机构地区：[1]School of Food Science and Technology,National Engineering Research Center of Seafood,Dalian Polytechnic University,Dalian 116034,China [2]Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China [3]Pharmacognosy Group,Department of Medicinal Chemistry,Uppsala University,Biomedical Centre,Uppsala 75123,Sweden

出　　处：《Food Science and Human Wellness》2023年第4期1192-1203,共12页食品科学与人类健康（英文）

基　　金：financially supported by the Project of Xingliao Talent Plan“Science and Technology Innovation Leader”(XLYC1802047).

摘　　要：There are many active substances in Atlantic cod(Gadus morhua)explaining the variety of biological activities.In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level.In this study,cod peptides were isolated by 80%ethanol extraction method,the isolated ethanol-soluble cod peptides(CP-ES)were investigated and their immunomodulatory activity was verified.Additionally,CP-ES showed lower molecular weight and more hydrophobic amino acids.CP-ES could promote the proliferation of spleen lymphocytes and T lymphocytes in mice,suggesting that CP-ES may regulate adaptive immunity.It promoted the release of NO and the expression of iNOS,TNF-α,IL-6 and IL-1βgenes in macrophages,suggesting that CP-ES may regulate innate immunity.CP-ES could promote the expression of TLR2 gene,and the peptides identified in CP-ES were docked with TLR2 to predict the peptides playing a major role in CP-ES.These results suggested that CP-ES may regulate the immune activity of both innate and adaptive lines.

关 键 词：Gadus morhua IMMUNOMODULATION PEPTIDES TLR2 Molecular docking 

分 类 号：TS201[轻工技术与工程—食品科学]