局部注射肝细胞生长因子基因修饰的骨髓间充质干细胞对兔前交叉韧带重建后腱骨愈合的影响  

Effect of local injection of hepatocyte growth factor transfected bone marrow mesenchymal stem cells on tendon-bone healing after anterior cruciate ligament reconstruction in rabbits

作　　者：杨德勇[1] 凯沙尔·百合提亚尔 姜钧耀 舒莉[1] YANG Deyong;Kaishaer Baihetiyaer;JIANG Junyao;SHU Li(The First Department of Joint Surgery,the Sixth Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi830002,China;Basic Medical College,Henan Science and Technology University,Henan Province,Luoyang471003,China)

机构地区：[1]新疆医科大学第六附属医院关节外一科,新疆乌鲁木齐830002 [2]河南科技大学基础医学院,河南洛阳471003

出　　处：《中国医药导报》2022年第35期28-31,共4页China Medical Herald

基　　金：新疆维吾尔自治区自然科学基金资助项目(2021 D01C453)。

摘　　要：目的探讨局部注射肝细胞生长因子(HGF)基因修饰的骨髓间充质干细胞(MSC)对兔前交叉韧带(ACL)重建后腱骨愈合的影响。方法45只3~5个月雄性新西兰兔(3.0~3.5 kg)采用随机数字表法分为A、B、C组,每组15只。三组均行双后肢ACL重建,A组ACL术后不注射任何物质;B组术后胫骨骨道内注射MSCs纤维蛋白胶;C组术后胫骨骨道内注射HGF基因修饰的MSCs纤维蛋白胶。术后6周,HE染色观察腱骨愈合;力学检测移植腱从胫骨骨道内脱出的最大拉力载荷及最大拉伸距离;RT-PCR检测骨道内成纤维细胞生长因子2(FGF2)mRNA、SMAD4 mRNA及关节液中HGF mRNA表达情况。结果术后6周,A组腱骨界面连接疏松,胶原纤维排列紊乱;B组腱骨界面连接较紧密,间隙较小,但胶原纤维排列仍不整齐;C组腱骨界面连接紧密,胶原纤维排列整齐。B组最大拉力载荷高于A组,且C组高于B组(P<0.05);A、B组及B、C组最大拉伸距离比较,差异无统计学意义(P>0.05)。B组骨道内FGF2 mRNA、SMAD4 mRNA大于A组,且C组大于B组(P<0.05);C组关节液HGF mRNA大于B组(P<0.05)。结论局部注射HGF基因修饰的MSCs能够促进兔ACL重建后腱骨愈合。Objective To explore the effect of hepatocyte growth factor(HGF)transfected bone marrow mesenchymal stem cell(MSC)on tendon-bone healing after anterior cruciate ligament(ACL)reconstruction in rabbits.Methods Forty-five male New Zealand rabbits(3.0-3.5 kg)aged three to five months were divided into groups A,B,and C by random number table method,with 15 rabbits in each group.All three groups underwent double hindlimb ACL reconstruction,group A did not inject any substance after ACL operation;in group B,MSCs fibrin glue was injected into the tibial canal after operation;in group C,HGF gene modified MSCs fibrin glue was injected into the tibial canal after operation.Six weeks after operation,HE staining was used to observe the tendon-bone healing;the maximum tensile load and the maximum stretching distance of the graft tendon exiting from the tibial canal were tested;the expression of fibroblast growth factor 2(FGF2)mRNA and SMAD4 mRNA in bone canal and HGF mRNA in joint fluid were detected by RT-PCR.Results Six weeks after operation,the tendon-bone interface of group A was loosely connected and collagen fibers were disordered;in group B,the tendon-bone interface was closely connected and the gap was small,but the arrangement of collagen fibers was not orderly;in group C,the tendon-bone interface was closely connected and the collagen fibers were neatly arranged.The maximum tensile load of group B was higher than that of group A,and that of group C was higher than that of group B(P<0.05);there was no significant difference in the maximum stretching distance between groups A,B and groups B,C(P>0.05).The FGF2 mRNA and SMAD4 mRNA in bone canal in group B were higher than those in group A,and those in group C were higher than those in group B(P<0.05);HGF mRNA of joint fluid in group C was higher than that in group B (P<0.05). Conclusion Local injection of HGF transfected bone marrow MSCs can enhance tendon-bone healing after ACL reconstruction in rabbits.

关 键 词：前交叉韧带 重建 腱骨愈合 肝细胞生长因子 间充质干细胞 

分 类 号：R686[医药卫生—骨科学]