前列消癥汤对前列腺癌PC-3细胞上皮-间充质转化的影响  

Effect of Qianlie Xiaozheng Decoction on epithelial-mesenchymal transformation of PC-3 cells in prostate cancer

作　　者：张奇[1] 于慧兰[1] 刘昭文 祁雷磊[1] 巩瑛杰 宋竖旗[1] ZHANG Qi;YU Huilan;LIU Zhaowen;QI Leilei;GONG Yingjie;SONG Shuqi(Department of Urinary Surgery,Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing100053,China)

机构地区：[1]中国中医科学院广安门医院泌尿外科,北京100053

出　　处：《中国医药导报》2022年第34期34-38,共5页China Medical Herald

基　　金：北京市自然科学基金资助项目(7192182)。

摘　　要：目的探讨前列消癥汤对前列腺癌PC-3细胞侵袭、迁移及上皮-间充质转化的影响。方法选取前列腺癌PC-3细胞作为研究对象,设置空白组、阳性药组(PC-SPESⅡ62.3μl/100 ml)及前列消癥汤低(62.5μl/100 ml)、中(125.0μl/100 ml)、高(250.0μl/100 ml)剂量组。采用侵袭、划痕实验观察五组细胞侵袭和迁移变化。采用蛋白质印迹法及PCR测定上皮-间充质转化相关蛋白与基因表达。结果前列消癥汤中、高剂量组侵袭细胞计数、迁移率低于空白组及前列消癥汤低剂量组,前列消癥汤高剂量组侵袭细胞计数低于前列消癥汤中剂量组,差异有统计学意义(P<0.05或P<0.01)。前列消癥汤低、中、高剂量组神经钙黏素蛋白表达低于空白组,前列消癥汤低、高剂量组SLUG蛋白表达低于空白组,前列消癥汤高剂量组SLUG蛋白表达低于前列消癥汤中剂量组,前列消癥汤高剂量组上皮钙黏素蛋白表达高于空白组及前列消癥汤低、中剂量组,差异有高度统计学意义(P<0.01)。前列消癥汤低、中剂量组SLUG、TWIST、神经钙黏素基因表达均低于空白组,前列消癥汤高剂量组上皮钙黏素基因表达高于空白组及前列消癥汤低、中剂量组,差异有统计学意义(P<0.05或P<0.01)。结论前列消癥汤可能通过调控PC-3细胞上皮-间充质转化抑制肿瘤细胞侵袭和迁移,延缓转移性前列腺癌的进展。Objective To investigate the effects of Qianlie Xiaozheng Decoction on invasion,migration,and epithelial-mesenchymal transformation of PC-3 cells in prostate cancer.Methods PC-3 cells of prostate cancer were selected as the research object,and the blank group,positive group(PC-SPESⅡ62.3μl/100 ml),and Qianlie Xiaozheng Decoction low(62.5μl/100 ml),medium(125.0μl/100 ml),and high(250.0μl/100 ml)dose groups were set.The invasion and migration of five groups of cells were observed by invasion and scratch test.Western blot and PCR were used to determine the expression of epithelial-mesenchymal transformation related proteins and genes.Results The invasion cell count and migration rate of Qianlie Xiaozheng Decoction medium and high dose groups were lower than those of the blank group and Qianlie Xiaozheng Decoction low dose group,and the invasion cell count of Qianlie Xiaozheng Decoction high dose group was lower than that of Qianlie Xiaozheng Decoction medium dose group,the differences were statistically significant(P<0.05 or P<0.01).The expressions of N-cadherin protein in Qianlie Xiaozheng Decoction low,medium,and high dose groups were lower than those in the blank group,the expressions of SLUG protein in Qianlie Xiaozheng Decoction low and high dose groups were lower than those in the blank group,the expression of SLUG protein in Qianlie Xiaozheng Decoction high dose group was lower than that in the Qianlie Xiaozheng Decoction medium dose group,the expression of E-cadherin protein in Qianlie Xiaozheng Decoction high dose group was higher than those in the blank group,and Qianlie Xiaozheng Decoction low and medium dose groups,the differences were highly statistically significant(P<0.01).The expressions of SLUG,TWIST,and N-cadherin genes in Qianlie Xiaozheng Decoction low and medium dose groups were lower than those in the blank group,the expression of E-cadherin gene in Qianlie Xiaozheng Decoction high dose group was higher than that in the blank group and Qianlie Xiaozheng Decoction low and medium dose groups

关 键 词：前列消癥汤 前列腺癌 PC-3细胞 上皮-间充质转化 

分 类 号：R737.2[医药卫生—肿瘤]