宣肺健脾方对慢性阻塞性肺疾病大鼠肺组织病理、气道杯状细胞及黏蛋白5AC的影响  

Effects of Xuanfei Jianpi Formula on lung tissue pathology,airway goblet cells and Mucin5AC in rats with chronic obstructive pulmonary disease

作　　者：李先莉 史利卿[2] 马建岭[2] 季坤[2] 温绍惠[2] 王梁敏[2] 李渊[2] 魏双颖 LI Xianli;SHI Liqing;MA Jianling;JI Kun;WEN Shaohui;WANG Liangmin;LI Yuan;WEI Shuangyin(The Second Clinical School of Medicine,Beijing University of Chinese Medicine,Beijing100029,China;Department of Respiratory Fever,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing100078,China)

机构地区：[1]北京中医药大学第二临床医学院,北京100029 [2]北京中医药大学东方医院呼吸热病科,北京100078

出　　处：《中国医药导报》2022年第34期8-12,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81903987);北京中医药大学东方医院“1166”人才培养项目(040204001001002012)。

摘　　要：目的观察宣肺健脾方对慢性阻塞性肺疾病(COPD)大鼠肺组织病理、气道杯状细胞及黏蛋白5AC(MUC5AC)的影响。方法将鼠龄10~12周,体重(230±20)g的40只Wistar雄性大鼠,按随机数字表法分为空白组、模型组、中药组、西药组,每组10只。除空白组正常饲养外,其余各组采用注入脂多糖加熏香烟方法制备COPD大鼠模型。造模完成后中药组给予宣肺健脾方(1 ml/100 g),西药组给予沐舒坦(1 ml/100 g),空白组及模型组给予等量0.9%生理盐水,连续14 d。各组进行苏木精-伊红(HE)染色观察肺组织病理,检测支气管肺泡灌洗液(BALF)中白细胞总数及分类,进行阿尔辛蓝-过碘酸希夫(AB-PAS)染色观察杯状细胞,行免疫组织化学法检测MUC5AC。结果空白组肺组织结构完整,未见炎症细胞浸润,模型组肺组织结构破坏及炎症反应明显,中药组肺组织结构及炎症反应较模型组改善。模型组BALF中白细胞总数、中性粒细胞数、淋巴细胞数高于空白组,差异有统计学意义(P<0.05);中药组BALF中白细胞总数、中性粒细胞数、淋巴细胞数低于模型组,差异有统计学意义(P<0.05)。空白组偶有少量杯状细胞,模型组杯状细胞增生高于空白组;中药组、西药组杯状细胞增生低于模型组。模型组相对着色面积高于空白组,差异有统计学意义(P<0.05);中药组相对着色面积低于模型组,差异有统计学意义(P<0.05)。模型组肺组织MUC5AC蛋白表达高于空白组,差异有统计学意义(P<0.05);中药组MUC5AC蛋白表达低于模型组,差异有统计学意义(P<0.05)。结论宣肺健脾方可能通过抑制杯状细胞化生,下调MUC5AC的表达,减少气道黏液高分泌;同时可减轻肺组织病理损害。Objective To observe the effects of Xuanfei Jianpi Formula on the lung tissue pathology,airway goblet cells and Mucin5AC(MUC5AC)in rats with chronic obstructive pulmonary disease(COPD).Methods Forty Wistar male rats aged 10 to 12 weeks and weighing(230±20)g were divided into blank group,model group,traditional Chinese medicine group,and western medicine group according to random number table method,with ten rats in each group.Except the normal feeding of the blank group,the other groups were treated with lipopolysaccharide injection and smoked cigarettes to prepare COPD rat models.After the modeling,the traditional Chinese medicine group was given Xuanfei Jianpi Formula(1 ml/100 g),the western medicine group was given Mucosolvan(1 ml/100 g),and the blank and the model groups were given 0.9%normal saline for 14 consecutive days.Hematoxylin and eosin(HE)staining was performed to observe lung tissue pathology,and the total number and classification of white blood cells in bronchoalveolar lavage fluid(BALF)were detected.Goblet cells were observed by Alcian blue periodic acid-Schiff(AB-PAS)staining,and MUC5AC was detected by immunohistochemistry.Results The lung structure of blank group was complete,no inflammatory cell infiltration was observed,the lung structure destruction and inflammatory reaction were obvious in the model group,and the lung structure and inflammatory reaction were improved in the traditional Chinese medicine group compared with the model group.The total number of white blood cells,neutrophils and lymphocytes in BALF in model group were higher than those in blank group,and the differences were statistically significant(P<0.05).The total number of white blood cells,neutrophils and lymphocytes in BALF in traditional Chinese medicine group were lower than those in model group,and the differences were statistically significant(P<0.05).There were a few goblet cells occasionally in blank group,and the goblet cell proliferation in model group was higher than that in blank group.The goblet cell prolifera

关 键 词：慢性阻塞性肺疾病 气道黏液高分泌 宣肺健脾方 杯状细胞 黏蛋白5AC 

分 类 号：R285.5[医药卫生—中药学]