尿酸酶缺失大鼠的血细胞和血液流变学变化  

Changes of Blood Cells and Hemorheology in Uricase-Deficient Rats

作　　者：万绪莲 李律宇 李丹[3] 罗光云 李宁 云宇[3] 段为钢 WAN Xulian;LI Lvyu;LI Dan;LUO Guangyun;LI Ning;YUN Yu;DUAN Weigang(Yunnan University of Chinese Medicine,Kunming 650500,China;The third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,China;Kunming Medical University,Kunming 650500,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南中医药大学第三附属医院,云南昆明650500 [3]昆明医科大学,云南昆明650500

出　　处：《云南中医学院学报》2022年第6期66-70,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省应用基础研究项目(202101AY070001-007,202101AZ070001-010,202101AZ070001-093)。

摘　　要：目的检测雄性尿酸酶缺失大鼠(Kunming-DY大鼠)的血细胞和血液流变学变化以完善其生物学资料。方法与同龄雄性野生型SD大鼠对照,用磷钨酸法检测尿酸酶缺失大鼠45 d日龄的血尿酸水平,记录体质量和出入量,断尾采血用全自动动物血细胞分析仪分析21项血细胞指标,腹主动脉采血用全自动动物血液流变仪检测23项血液流变学指标。结果45 d日龄尿酸酶缺失大鼠的血尿酸水平明显升高,饮水量和尿量明显增加;白细胞数量减少,但淋巴细胞比例增加,红细胞体积变小,血小板体积也变小;多项血液流变学指标也有所改善。结论尿酸酶基因缺失大鼠具有慢性感染、营养性贫血、小细胞低色素特征的“贫血”倾向,血小板功能更倾向于成熟,有利于止血,血液黏度也有所下降。Objective To examine the changes of blood cells and hemorheology in male uricase-deficient rats(Kunming-DY rats)to improve and perfect its biological data.Methods Compared with male wild-type SD rats of the same age,serum uric acid(SUA)in uricase-deficient rats of 45-day age was determined using the phosphotungstic acid method.Their weight,intake and excreta were recorded.Their tail blood was collected in which the blood cells were analyzed by an automatic animal blood cell analyzer,and 21 results were obtained.The blood from their abdominal aorta was drawn whose 23 hemorheological indexes were analyzed by an automatic animal blood rheometer.Results The SUA level,intake of water and urine volume of uricase-deficient rats of 45-day age were significantly increased.The number of white blood cells decreased with the proportion of lymphocytes increasing,and red blood cell volume decreased along with platelet volume decreasing.In addition,several hemorheological indexes were improved.Conclusion The uricase-deficient rats are prone to infection,nutritional anemia,and"anemia"characterized by small cell low pigment.The platelet function is more mature,which is conducive to hemostasis,and the blood viscosity is also reduced.

关 键 词：尿酸酶缺失大鼠 高尿酸血症 血细胞分析 血液流变学 Kunming-DY大鼠 大鼠模型 

分 类 号：R-332[医药卫生]