Metabolic Labeling and Imaging of Cellular RNA via Bioorthogonal Cyclopropene−Tetrazine Ligation  

作　　者：Zhiyong He Shuang Peng Qi Wei Shaokang Jia Shan Guo Kun Chen Xiang Zhou 

机构地区：[1]The College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers of Ministry of Education,Wuhan University,Wuhan 430072

出　　处：《CCS Chemistry》2020年第3期89-97,共9页中国化学会会刊（英文）

基　　金：supported financially by the National Natural Science Foundation of China(21432008,91753201,and 21721005).

摘　　要：RNA labeling is vital for the study of an RNA structure,cellular distribution,localization,and metabolism.Herein,we report N6 cyclopropane-modified adenosine(cpA)as a new analog for metabolic RNA labeling.We successfully applied inverse electrondemand Diels–Alder(iEDDA)chemistry to label cellular RNA with cpA.This labeling technique is practical and provides a new platform to study RNA roles in cells in a metal-free manner.This simple and robust assay represents a significant advancement in the profiling methods of the nascent transcriptome using chemical approaches.

关 键 词：bioorthogonal chemistry Diels–Alder cycloaddition RNA metabolic labeling modified nucleoside cell imaging 

分 类 号：O6[理学—化学]