基于微流控芯片的复方木鸡颗粒抗肝肿瘤的“谱-物-效”关系  

Study on“Spectrum-material-effect”relationship of Compound Muji Granules against liver tumor based on microfluidic chip

作　　者：秦妍 包永睿 王帅 李天娇 张秀君 孟宪生 潘海鸥 QIN Yan;BAO Yong-rui;WANG Shuai;LI Tian-jiao;ZHANG Xiu-jun;MENG Xian-sheng;PAN Hai-ou(College of Traditional Chinese Medicine,Liaoning University of Traditional Chinese Medicine;Liaoning Professional Innovation Technology Center for Multidimensional Analysis of Traditional Chinese Medicine,Liaoning Modern Traditional Chinese Medicine Research Engineering Laboratory,Dalian Liaoning 116600,China;Dandong Pharmaceutical Group Co.,Ltd,Dandong Liaoning 118303,China;College of Foreign Languages,Liaoning University of Traditinal Medicine,Shenyang Liaoning 110000,China)

机构地区：[1]辽宁中医药大学药学院 [2]辽宁省中药多维分析专业创新技术中心,辽宁省现代中药研究工程实验室,辽宁大连116600 [3]丹东药业集团有限公司,辽宁丹东118303 [4]辽宁中医药大学外国语学院,辽宁沈阳110000

出　　处：《中国药理学通报》2023年第1期185-192,共8页Chinese Pharmacological Bulletin

基　　金：民族创新联合基金项目(No2020-MZLH-35)。

摘　　要：目的探讨复方木鸡颗粒抗肝肿瘤作用的物质基础。方法采用微流控芯片技术对复方木鸡颗粒进行体外抗肝肿瘤药效学研究;采用HPLC法建立复方木鸡颗粒指纹图谱;采用灰色关联度分析、偏最小二乘回归以及网络药理学方法,对复方木鸡颗粒进行“谱-物-效”分析;采用UPLC-Q-TOF-MS技术对药效成分进行解析;结果成功建立7批不同提取方式的复方木鸡颗粒指纹图谱,灰色关联分析结果显示复方木鸡颗粒与药效关联系数>0.80的14个成分中有7个呈正相关;对抗肝肿瘤具有促进作用的贡献依次为峰号48(木犀草素)>6(没食子酸)>19(绿原酸)>59(槲皮素)>67(山柰酚)>65(柚皮素)>38(鞣花酸)。结论本实验通过建立“谱-物-效”研究方法,明确以上7种活性单体很可能为复方木鸡颗粒的抗肝肿瘤物质基础。Aim To explore the material basis of anti-tumor effect of Compound Muji Granules.Methods The anti-tumor pharmacodynamics of Compound Muji Granules in vitro was studied by microfluidic chip technology.The fingerprint of Compound Muji Granules was established by HPLC.The“Spectrum-Material-Effect”of Compound Muji Granules was analyzed by grey correlation analysis,partial least squares regression analysis and network pharmacology approach.Results Seven batches of Compound Muji Granules with different extraction methods were successfully established.The results of grey correlation analysis showed that there was a positive correlation between Compound Muji Granules and 7 of the 14 components with pharmacodynamic correlation coefficient>0.80.The contribution of anti liver tumor was peak number 48(luteolin)>6(gallic acid)>19(chlorogenic acid)>59(quercetin)>67(kaempferol)>65(naringin)>38(ellagic acid),in that order.Conclusions Through the establishment of“Spectrum-Material-Effect”research method,it is clear that the above seven active monomers may be the anti-tumor material basis of Compound Muji Granules.

关 键 词：复方木鸡颗粒 肝肿瘤 指纹图谱 微流控芯片 谱效分析 物质基础 

分 类 号：R282.71[医药卫生—中药学] R735.7[医药卫生—中医学] R979.1