用IRE_FINDER算法在非翻译区搜索人和小鼠的铁反应元件  

IRE_FINDER——Computational Search of Iron Response Element in Human and Mouse UTRs

作　　者：陈新[1] 王鲁泉 黄弋[1] 邱平 MURGOLO Nicholas J. GREENE Jonathan R. 吴才宏[3] 姜颖[1] 

机构地区：[1]北京大学生命科学学院生物信息中心,北京100871 [2]Schering Plough研究所,nj07033 [3]北京大学膜及膜的工程国家重点实验室,北京100871

出　　处：《生物化学与生物物理学报》2002年第6期743-747,共5页

基　　金：国家高技术研究发展计划资助项目 (863计划 ) (No .2001AA231011)~~

摘　　要：铁反应元件 (IRE)具有的高度保守的RNA茎环结构 ,是胞质中IRE结合铁调控蛋白 (IRP)的位点。IRP与IRE的结合受细胞内铁含量的调控 ,与铁代谢有着密切的关系。许多与铁代谢有关的基因在 5′UTR或 3′UTR都存在IRE。当铁离子缺乏时 ,IRE与IRP结合 ,使核糖体的翻译进程受阻 ,抑制mRNA的翻译 ;而当铁离子浓度较高时 ,IRP就释放IRE ,促进翻译。因此 ,IRE在 5′UTR调节着翻译的起始[1] 。如果IRE位于 3′UTR ,IRE与IRP的结合可以稳定mRNA并预防其被降解。所有已知的IRE均为C1G5型 ,即环的 1号和 5号碱基位置分别是保守的C和G ;另外还存在一类体外合成的U1A5型IRE ,即环的 1号和 5号碱基位置分别是保守的U和A ,能够在体外有效地结合IRP。由于IRE与铁代谢、氧化性损伤 (oxidativestress)和衰老等过程都有密切的关系 ,因此在临床及新药研制等方面都很受重视。通过改进搜索IRE的算法 ,用计算机程序对人与鼠的mRNA非编码区(UTR)序列数据库进行全面的搜索 ,期望找出可能存在的C1G5或U1A5类型的IRE ,或包含这两种类型IRE的基因。The iron response element (IRE) is a highly conserved RNA stem loop structure. It is the binding site of iron regulatory protein (IRP). IRP binding to IRE is regulated by cellular iron. When cells are derived of iron, IRP binds IRE. If IRE is located at 5′UTR, IRP binding will inhibit translation initiation, else if IRE is at 3′UTR, IRP binding will stabilize mRNA and prevent it from degradation. So far all known IREs have C at the 1 position and G at the 5 position of the loop (C1G5 type).In vitro studies suggest that the U1A5 type IRE, which has U and A at the 1 and 5 loop position respectively, binds well to IRP. However, U1A5 type'sin vivo existence is still elusive. IRE-IRP binding is involved in the regulation of iron metabolism, oxidative stress and possibly aging. Here we use an improved computation method performing a comprehensive search of IRE in human and mouse genes. We try to catalog potential human and mouse IRE containing genes, at the same time identify potential U1A5 IREs.

关 键 词：IRE-FINDER算法 非翻译区 搜索 人 小鼠 铁反应元件 铁调控蛋白 茎环结构 

分 类 号：Q753[生物学—分子生物学]