单细胞转录组测序技术及在哺乳动物上的应用  

Application of Single-cell Transcriptome Sequencing in Mammalian

作　　者：寇佳怡 王玉玲 曾睿琳 兰道亮[2,3] KOU Jia-yi;WANG Yu-ling;ZENG Rui-lin;LAN Dao-liang(Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041;School of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041;Ministry of Education of Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization,Southwest Minzu University,Chengdu 610041)

机构地区：[1]西南民族大学青藏高原研究院,成都610041 [2]西南民族大学畜牧兽医学院,成都610041 [3]青藏高原动物遗传资源保护与利用教育部重点实验室,成都610041

出　　处：《生物技术通报》2022年第11期41-48,共8页Biotechnology Bulletin

基　　金：西南民族大学中央高校基本科研专项资金资助项目(2021NYYXS93)。

摘　　要：哺乳动物的器官是由多种细胞类型组成,它们通过细胞间的相互作用来发出信号,以维持体内平衡和确保机体发育。传统转录组测序是以大量细胞或组织为研究样本,反映的是细胞总体上转录组特征,不能分析单个细胞的基因表达情况,而单细胞RNA测序(single-cell RNA sequencing,scRNA-seq)技术的发展为揭示单个细胞转录组特征提供了有效方法。本文通过对scRNA-seq平台、scRNA-seq主要技术类型及scRNA-seq在哺乳动物上的应用展开综述。Mammalian organs are composed of many cell types.They send signals through cell-cell interactions to maintain homeostasis and ensure body development.Traditional transcriptome sequencing takes a large number of cells or tissues as research samples,reflecting the overall transcriptome characteristics of cells,but cannot analyze the gene expression of a single cell.The development of single cell RNA sequencing(scRNA-seq)provides an effective method for revealing the characteristics of single cell transcriptome.In this paper,the scRNA-seq platform,the main types of scRNA-seq technologies and the applications of scRNA-seq in mammals are reviewed.

关 键 词：单细胞转录组测序 哺乳动物 细胞类型 异质性 

分 类 号：Q811.4[生物学—生物工程]