慈竹纤维素合酶BeCesA4的克隆及功能分析  

Cloning and Functional Analysis of BeCesA4 in Bambusa emeiensis

作　　者：王博雅 姜勇 黄艳[1] 曹颖[1] 胡尚连[1] WANG Bo-ya;JIANG Yong;HUANG Yan;CAO Ying;HU Shang-lian(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010

出　　处：《生物技术通报》2022年第11期185-193,共9页Biotechnology Bulletin

基　　金：突破性竹类及花卉育种材料和方法创新及新品种选育(2021YFYZ0006)。

摘　　要：非木造浆是解决国内纸浆短缺的重要手段。慈竹(Bambusa emeiensis)是我国非木造浆的主要原材料之一,提高慈竹中纤维素的含量能够有效提高竹类造浆的效率。通过前期对慈竹进行的转录组测序分析,挖掘出慈竹中一个与植物中纤维素合酶亚基A(cellulose synthase A,CesA)同源的基因,命名为BeCesA4。结果显示,克隆出的BeCesA4基因编码一个含有982个氨基酸的蛋白质,具备CesA家族的保守结构域;BeCesA4在慈竹快速生长的笋与茎中显著表达;过量表达该基因会使转基因植物出现生物量提升、纤维素含量升高和次生细胞壁加厚等现象。结果表明,BeCesA4的表达量与慈竹茎内纤维素的积累呈正相关。本研究结果为进一步揭示慈竹纤维素合成机制奠定了基础。Producing paper pulp by non-wood species is an important way to solve the shortage of domestic pulp.Bambusa emeiensis is one of major bamboo species in non-wood pulp-making industry,thus increasing the cellulose content of B.emeiensis would effectively increase the pulp producing efficacy from bamboo.Based on RNA-seq analysis of B.emeiensis in our early work,we have found a gene that is highly homologous to known plant cellulose synthase A and named it as BeCesA4.The results showed that the cloned BeCesA4 encoded a protein containing 982 amino acids and had a conserved CesA domain.BeCesA4 mostly expressed in fast-growing organs like shoot and stem of B.emeiensis.Overexpression of BeCesA4 resulted in the biomass accumulation,higher cellulose content and thicker secondary cell wall of stem in transgenic plants.Thus,the expression of BeCesA4 is positively corrected with the accumulation of cellulose in B.emeiensis.These results lay a foundation for revealing the synthesis mechanism of cellulose in B.emeiensis.

关 键 词：慈竹 纤维素合酶 次生壁 异源表达 

分 类 号：S795.5[农业科学—林木遗传育种] Q943.2[农业科学—林学]