氮添加对樟子松人工林土壤细菌磷酸酶编码基因丰度的影响  

Effects of Nitrogen Addition on the Abundance of Bacterial Phosphatase Encoding Genes in the Soil of Pinus sylvestris var.mongolica Plantation

作　　者：武林辉 耿必苗 王艳杰 周国伟 孙庆业[1] 赵琼 WU Lin-hui;GENG Bi-miao;WANG Yan-jie;ZHOU Guo-wei;SUN Qing-ye;ZHAO Qiong(College of Resources and Environmental Engineering,Anhui University,Hefei 230601)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601

出　　处：《生物技术通报》2022年第11期202-209,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(41877341)。

摘　　要：为研究氮添加影响森林土壤有机磷矿化的微生物调控机制,分析了10年的野外氮添加(100 kg N^(-2) ha^(-2)year^(-1))对沙地樟子松人工林土壤微生物中编码酸性磷酸单酯酶、碱性磷酸单酯酶和植酸酶的功能基因(phoC、phoD和appA)丰度及相关酶活性和土壤理化性质的影响。结果表明,氮添加使樟子松人工林土壤中酸性和碱性磷酸单酯酶活性分别下降了18.09%和55.29%,植酸酶活性下降了41.88%。氮添加使土壤微生物中各基因拷贝数分别下降40.97%(16S-rRNA)、78.38%(phoD)、67.92%(phoC)、74.37%(appA)。各基因拷贝数占总细菌基因拷贝数的比例显著下降了61%(phoD)、44%(phoC)、55%(appA)。土壤微生物量碳、微生物量磷含量与酸性磷酸单脂酶、碱性磷酸单脂酶、植酸酶活性及16S rRNA、phoD、phoC、appA基因丰度显著正相关。土壤铵态氮含量与酸性磷酸单脂酶、碱性磷酸单脂酶活性及16S rRNA、phoC、appA基因丰度显著负相关。酸性磷酸单酯酶活性与其基因丰度显著正相关,其他两种酶活性与其基因丰度无显著相关性。以上结果表明,氮添加不仅降低了土壤解磷微生物丰度,还降低了解磷细菌在总细菌中的比例,从而大大降低了磷酸酶活性,抑制了有机磷的矿化。In order to reveal the microbial processes regulating the responses of soil organic phosphorus(P)mineralization to nitrogen(N)addition,abundances of 3 soil bacterial phosphatase-encoding genes(phoC,phoD and appA)and corresponding acid phosphomonoesterase,alkaline phosphomonoesterase and phytase activities,as well as related soil physiochemical properties were measured in a Pinus sylvestris var.mongolica plantation that has subjected to 10 years of field N addition(100 kg N^(-2) ha^(-2)year^(-1)).The results show that N addition reduced acidic and alkaline phosphomonoesterase activities in the soil of Pinus sylvestris var.mongolica plantation by 18.09% and 55.29%,and the phytase activity decreased by 41.88%.Nitrogen addition reduced the copy number of each gene by 40.97%(16S-rRNA),78.38%(phoD),67.92%(phoC),and 74.37%(appA),respectively.The proportion of the copy number of functional gene in the total bacterial gene 16S-rRNA decreased significantly by 61%(phoD),44%(phoC),and 55%(appA).The contents of soil microbial biomass carbon and microbial biomass phosphorus were significantly positively correlated with the activities of acid phosphomonolipase,alkaline phosphomonoesterase,phytase and gene abundances of 16S rRNA,phoD,phoC and appA.Soil ammonium nitrogen content was significantly negatively correlated with the activities of acid phosphomonoesterase and alkaline phosphomonoesterase,as well as the abundance of 16S rRNA,phoC and appA genes.The activity of acid phosphomonoesterase was significantly positively correlated with its gene abundance,and the other two enzyme activities had no significant correlation with its gene abundance.The above results indicate that the N addition not only reduced abundance of soil phosphate-dissolving bacteria,but also reduced the proportion of phosphate-dissolving bacteria in the total bacteria,thereby greatly reduced phosphatase activities and depressed organic phosphorus mineralization.

关 键 词：氮添加 磷酸单酯酶 植酸酶 基因丰度 

分 类 号：S791.253[农业科学—林木遗传育种] S714.3[农业科学—林学]