一株高效溶磷细菌的条件优化及其溶磷特性研究  

Condition Optimization of an Efficient Phosphate-dissolving Bacterial Strain and Its Phosphate-dissolving Characteristics

作　　者：李思思 张博源 符运会 周佳[1] 屈建航[1] LI Si-si;ZHANG Bo-yuan;FU Yun-hui;ZHOU Jia;QU Jian-hang(College of Bioengineering,Henan University of Technology,Zhengzhou 450001)

机构地区：[1]河南工业大学生物工程学院,郑州450001

出　　处：《生物技术通报》2022年第12期274-286,共13页Biotechnology Bulletin

基　　金：河南省高等学校重点科研项目(20A180009);国家自然科学基金面上项目(42107139);河南工业大学创新基金支持计划专项(2021ZKCJ15)。

摘　　要：探究一株自湖泊沉积物中分离到的高效溶磷菌株1616X1的溶磷条件和特性。通过菌落形态、生理生化特性及16S rRNA基因序列分析进行菌种分类鉴定,采用钼锑抗比色法测定溶磷量,利用单因素和响应面试验对溶磷条件进行优化,土壤溶磷试验观察土壤中速效磷含量变化以及菌株1616X1在土壤中的定殖效果。菌株1616X1为Pseudomonas sp.;在NBRIP液体培养基中溶磷量与p H呈显著负相关,最佳溶磷条件为葡萄糖10 g/L、硫酸铵0.17 g/L、pH 5.70、接种量5%、装液量75 mL/250 mL,培养温度23.3℃,该条件下溶磷量为684.91 mg/L;1616X1在实验所用菜地以及花圃土壤中均能有效定殖且能显著增加土壤速效磷含量。菌株1616X1具有较好的溶磷能力,有望为开发溶磷微生物肥料提供优质菌种资源,为该溶磷菌的合理应用提供理论依据。In order to study the phosphate-dissolving conditions and characteristic of a highly effective phosphate-dissolving strain 1616X1 isolated from lake sediment, the classification and identification of bacterial species were carried out by colony morphology, physiological and biochemical characteristics and 16S rRNA gene sequence analysis. The method of Mo-Sb colorimetry was applied to measure the amount of dissolved phosphorus. The single factor and response surface experiment were applied to find optimal phosphate-dissolving condition. Through soil culture experiments, the changes of available phosphorus and the colonization of strains were observed. The result of identification revealed that phosphate-dissolving strain 1616X1 can be identified as Pseudomonas sp. The phosphate-dissolving ability of strain 1616X1 showed a negative correlation with p H in NBRIP liquid medium. The optimal phosphate-dissolving conditions were as follows :glucose 10 g/L, ammonium sulfate 0.17 g/L, pH5.70,inocula concentration 5.0%,loaded liquid 75 m L/250 mL, and fermentation temperature 23.3℃,under which the maximum phosphate-dissolving capacity was up to 684.91 mg/L. Concurrently,1616X1 effectively colonized in the experimental vegetable field and flowerbed soils, and significantly increased soil available phosphorus content. Strain 1616X1 presents a good ability of dissolving phosphorus, aiming to provide high-quality bacterial resources for the development of phosphate-dissolving microbial fertilizers, and to provide a theoretical basis for the rational application of the phosphate-dissolving bacteria.

关 键 词：溶磷 条件优化 响应面 土壤定殖 速效磷 

分 类 号：S154.3[农业科学—土壤学]