植物中C-糖基转移酶的结构与应用  

Structure and Application of C-glycosyltransferases in Plants

作　　者：赵玉雪 王芸[2] 余璐瑶 刘京晶[1] 斯金平[1] 张新凤[1] 张磊 ZHAO Yu-xue;WANG Yun;YU Lu-yao;LIU Jing-jing;SI Jin-ping;ZHANG Xin-feng;ZHANG Lei(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300;Biomedical Innovation R&D Center,School of Medicine,Shanghai University,Shanghai 200444;Department of Pharmaceutical Botany,School of Pharmacy,Naval Medical University,Shanghai 200433)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,杭州311300 [2]上海大学医学院生物医药创新研发中心,上海200444 [3]海军军医大学药学院药用植物学教研室,上海200433

出　　处：《生物技术通报》2022年第10期18-28,共11页Biotechnology Bulletin

基　　金：国家重点研发计划项目(2017YFC1702201);国家自然科学基金项目(31970316,32000231,32170274);上海市优秀学术带头人计划(19XD1405000)。

摘　　要：糖基转移酶是一类专门催化糖基化反应的后修饰酶,根据与糖供体连接的受体分子不同,分为O-、C-、N-、S-四种糖基转移酶。近年来,伴随着结构生物学的不断发展,关于C-糖基转移酶的结构解析及定向改造的报道日益增多,为复杂天然产物修饰提供理论依据。本文综述了植物C-糖基转移酶的研究进展,从其底物杂泛性、糖供体结合的空间结构与生物技术应用的角度进行讨论,探究造成底物催化特征的结构基础,为后续通过合成生物学手段异源合成植物天然产物提供参考。Glycosyltransferases are a class of post-modification enzymes that specifically catalyse glycosylation reactions and are classified into 4 types of glycosyltransferases,O-,C-,N-and S-,depending on the acceptor molecule attached to the sugar donor.In recent years,along with the vigorous development of structural biology,reports on the structural elucidation and targeted modification of C-glycosyltransferases have gradually increased,providing theoretical guidance for the modification of complex natural products.This paper reviews the research progress of plant C-glycosyltransferases,discusses them from the perspectives of their substrate specificity,spatial structure of sugar donor binding and biotechnological applications,and explores the structural basis of the substrate catalytic characteristics,which may provide a reference for the subsequent heterologous synthesis of plant natural products by means of synthetic biology.

关 键 词：C-糖基转移酶 生物合成途径 催化机制 晶体结构 合成生物学 

分 类 号：Q946[生物学—植物学]