植物miR396-GRF模块的生物学功能及其潜在应用价值  

Research Progress in Biological Functions of miR396-GRF Module in Plants and Its Potential Application Values

作　　者：山琦 贾惠舒 姚文博 刘伟灿[1] 李海燕[2] SHAN Qi;JIA Hui-shu;YAO Wen-bo;LIU Wei-can;LI Hai-yan(College of Life Sciences,Jilin Agricultural University,Changchun 130118;College of Tropical Crops,Hainan University,Haikou 570228)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]海南大学热带作物学院,海口570228

出　　处：《生物技术通报》2022年第10期34-44,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(32001464)。

摘　　要：miR396是植物中一种保守的microRNA,生长调节因子(growth regulatory factor,GRF)基因已在多个物种中被证实是其作用的主要靶基因。目前的研究报道显示,miR396介导的靶基因GRF调控途径(miR396-GRF),已在利用分子育种技术进行植物品种改良,及提升植物组织材料再生效率方面展露出了诱人的应用潜力。本文分析了miR396和GRF基因的序列特点;阐明了miR396-GRF模块的具体互作模式;并着重介绍了近年来miR396-GRF模块在调控植物生长发育、逆境胁迫响应和影响植物组织再生效率方面的生物学功能研究进展,也收集了miR396-GRF模块提高植物生物量及作物产量、改善植物逆境胁迫耐受能力及提高植物材料在遗传转化过程中再生效率方面的研究案例;最后,总结了目前关于miR396-GRF模块发挥生物学功能的分子机制研究概况,旨为进一步深入研究miR396-GRF途径提供思路和参考。miR396 is a conserved microRNA in plant,the growth regulatory factor(GRF)gene has been proved to be their main target genes in many plant species.Current reports indicate that miR396-mediated GRF regulatory pathway(miR396-GRF)has shown an attractive application potential in improving plant varieties by molecular breeding and and promoting the regeneration efficiency of plant tissue materials.In this paper,the sequence characteristics of miR396 and GRF genes were analyzed,and the specific interaction mode of miR396-GRF modules was elucidated.In addition,the biological functions of miR396-GRF module in plant growth and development,stress response and plant tissue regeneration were reviewed.The research events of miR396-GRF module in improving plant biomass and crop yield,plant tolerance to stress and plant regeneration efficiency during genetic transformation were collected.And the current research on the molecular mechanism of miR396-GRF module were summarized.To sum up,this paper provides ideas and references for further study of miR396-GRF pathway.

关 键 词：miR396 GRF 逆境胁迫 产量 转化效率 

分 类 号：Q943.2[生物学—植物学]