玉米NF-Y转录因子基因ZmNF-YB13响应干旱和盐胁迫的功能分析  

Functional Analysis of ZmNF-YB13 Responding to Drought and Salt Stress

作　　者：张彤彤 郑登俞 吴忠义[2] 张中保[2] 于荣[1] ZHANG Tong-tong;ZHENG Deng-yu;WU Zhong-yi;ZHANG Zhong-bao;YU Rong(College of Life Sciences,Capital Normal University,Beijing 100048;Institute of Biotechnology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097)

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京市农林科学院生物技术研究所,北京100097

出　　处：《生物技术通报》2022年第10期115-123,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31871351);北京市农林科学院创新能力建设专项(KJCX20200205,KJCX20200407)。

摘　　要：玉米NF-Y家族是一类重要的转录因子,在调控植物发育和逆境胁迫响应中起重要作用,探究该家族基因功能将为玉米抗逆育种提供重要的基因资源。本研究克隆获得ZmNF-YB13基因,使用生物信息学、实时荧光定量PCR等技术对该基因的基本特性、组织表达特性及响应逆境胁迫表达模式等进行分析。结果显示,该基因全长537 bp,编码蛋白含有178个氨基酸,分子量为18.9 kD,理论等电点为6.83,具有NF-Y家族特有的保守结构域。qPCR分析表明,ZmNF-YB13基因在玉米花丝中的表达量最高;同时ZmNF-YB13基因在不同逆境胁迫以及激素处理条件下均有不同程度的上调表达。在分别含有甘露醇、NaCl、ABA和JA的1/2 MS培养基上,转ZmNF-YB13基因拟南芥的根长于野生型,差异显著。在干旱和高盐处理下,土壤中生长的转基因拟南芥比野生型绿叶数多,MDA含量低,差异显著;同时干旱处理下,转基因植株的POD活性显著高于野生型。由此推测,ZmNFYB13基因可能参与玉米耐旱、抗盐非生物胁迫的应答。Maize NF-Y family is a class of important transcription factors,they play an important role in regulating plant development and stress response.Exploring the gene function of this family will provide important gene resources for maize stress-resistant breeding.In this study,ZmNF-YB13 gene was cloned,and its basic characteristics,the relative tissue-specific expressions and expression patterns under different stresses were analyzed by bioinformatics and real-time quantitative PCR.The gene was 537 bp in length and encoded 178 amino acids.The molecular weight of ZmNF-YB13 protein was 18.9 kD,and the theoretical isoelectric point was 6.83.ZmNF-YB13 protein had a unique conserved domain of NF-Y family.qPCR analysis showed that ZmNF-YB13 gene expression was the highest in maize silk;meanwhile ZmNFYB13 gene expression was up-regulated under different abiotic stress and hormone stress.On 1/2 MS medium containing mannitol,NaCl,ABA and JA,the root length of ZmNF-YB13 transgenic Arabidopsis thaliana was significantly longer than that of the wild type.Under drought and high salt treatments,transgenic A.thaliana grown in soil had more green leaves and lower MDA content than wild-type.Concurrently,the POD activity of transgenic plants was significantly higher than that of wild type under drought treatment.It is speculated that ZmNF-YB13 gene may be involved in response to drought and salt stress in maize.

关 键 词：玉米 转录因子 异源表达 干旱胁迫 盐胁迫 

分 类 号：S513[农业科学—作物学] Q943.2[生物学—植物学]