三系杂交小麦混播制种对杂交种产量、纯度及F_(1)产量优势的影响  

Effects of Blend Seeding on the Yield,Purity and Yield Advantage of F_(1) in Three-line Hybrid Wheat

作　　者：孔德真 聂迎彬[1] 徐红军[1] 崔凤娟[1] 穆培源[1] 田笑明[1] KONG De-zhen;NIE Ying-bin;XU Hong-jun;CUI Feng-juan;MU Pei-yuan;TIAN Xiao-ming(Institute of Crop Research,Xinjiang Academy of Agri-Reclamation Sciences/Key Lab of Xinjiang Production and Construction Corps for Cereal Quality Research and Genetic Improvement,Shihezi 832000)

机构地区：[1]新疆农垦科学院作物研究所/谷物品质与遗传改良兵团重点实验室,石河子832000

出　　处：《生物技术通报》2022年第10期132-139,共8页Biotechnology Bulletin

基　　金：兵团重点领域科技攻关(2018AB040)。

摘　　要：为探讨杂交小麦强优势组合制种混播不同比例恢复系对制种产量、纯度及F_(1)代产量的影响,以3个AL(Alborubrum Korn)型CMS(cytoplasmic male sterility)小麦杂交组合为材料,在不育系(母本♀)种子中混入不同比例的恢复系(父本♂)种子播种,研究了杂交制种的产量、纯度和F_(1)代产量优势变化。结果表明:组合36A×2014AR5和002A×99AR142-1在混播不同比例恢复系制种时,混播混收制种产量随父本混入比例增加而提高,相应杂交种的种子纯度逐渐降低。当父本的混播比例为5%时,可生产出纯度为96%的杂交种,混播混收制种产量也较高;对2个杂交组合不同混播比例制种的F_(1)代进行产量比较,混播混收制种产量均高于同组合行比制种的杂交种对照,混播比例分别为12%和9%时的F_(1)代产量表现更显著;利用与位于染色体2A上的恢复基因(qRf-2A-1)连锁的SSR分子标记Wmc474检测,预测了不同混入比例下混播混收杂交种的种子纯度,同时与前期位于1B染色体上的恢复基因(qRf-1B-1)连锁的SSR标记Xbarc8建立的AL型三系杂交小麦杂交种纯度检测回归方程预测结果进行了比较,表明用2个分子标记建立的2个回归方程在混播恢复系制种的纯度鉴别中均可使用。In order to explore the effects of different ratios of restoring lines on seed production,purity and heterosis of F_(1) yield in hybrid wheat production with strong heterosis combinations,three AL(Alborubrum Korn)-type CMS(cytoplasmic male sterility)wheat hybrid combinations were used as materials in the sterile line(female parent♀).The seeds were mixed with different proportions of restoring line(male parent♂)seeds for sowing.The yield of hybrid seed production,the purity of seed production,and the change of hybrid F_(1) yield heterosis were studied.The results showed that the two hybrid combinations 36A×2014AR5 and 002A×99AR142-1 were mixed with different proportions of restoration line seed production,the yield of hybrid seed production increased with the increase of the mixed male parent ratio,and the seed purity of the hybrids gradually decreased.When the male parent’s mixed sowing proportion was 5%,it reached the requirement of 96%of field seed purity,and the seed production yield was also higher.The yield of the F_(1) generations produced by the two hybrid combinations with different mixed sowing ratios was compared,and the mixed seeding production.The seed yields of mixed sowing and mixed harvesting were higher than those of the hybrid control of the same combination and row ratio seed production,and the yield advantage of F_(1) with mixing ratios of 12%and 9%was more significant.The SSR molecular markers Wmc474,which were linked to the restorer gene(qRf-2A-1)and located on chromosomes 2A,were used to predict the seed purities via mixed sowing and mixed harvesting under different mixing ratios.Concurrently,the prediction results of the regression equation for purity detection of AL-type three-line hybrid wheat hybrids established by the SSR marker Xbarc8 linked to the restorer gene(qRf-1B-1)on chromosome 1B were compared,showing that the two regression equations established with the two molecular markers can be used in the identification of the purity of the seed production of mixed restorer lines

关 键 词：杂交小麦 混播混收制种 纯度检测 分子标记 

分 类 号：S512.1[农业科学—作物学]