大麦Thionin-like基因家族基因表达谱分析  

Expression Profile Analysis of Thionin-like Gene Family in Barley

作　　者：杨宏亮 袁桢 钱徐佳志 徐大伟 YANG Hong-liang;YUAN Zhen;QIAN Xu-jia-zhi;XU Da-wei(School of Agronomy,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学农学院,合肥230036

出　　处：《生物技术通报》2022年第10期140-147,共8页Biotechnology Bulletin

基　　金：国家自然科学基金青年项目(31900611)。

摘　　要：类硫素基因Thil(Thionin-like)在植物防御系统中具有抗病原微生物的活性,然而对它的功能却不清楚。通过对Thil基因家族在大麦中的序列组成及基因结构、染色体分布、蛋白保守结构域、系统进化树、基因启动子元件、亚细胞定位和表达谱分析,为其生物学功能研究提供借鉴。共鉴定出7个大麦Thil基因,这些基因分布于1、2、4、5和6号染色体上;基因结构分析均表明,除Thil4存在一定的差异外,其它家族成员序列都较为保守;通过系统进化树可以看出大麦Thil基因分为4个亚家族;表达谱分析结果显示,在大麦不同组织中,Thil基因的表达量有所不同,总体来看,Thil基因在根和种子中的表达量极低,在幼叶中表达量最高。Thil家族不同成员之间的蛋白亚细胞定位结果也不一样,Thil1和Thil2主要定位于细胞膜、细胞核和细胞质上,Thil3和Thil7定位于细胞膜上,Thil6定位于叶绿体。Thil基因与植物的保护和防御机制有关,在幼叶中能够激发植物保护机制的元件响应最多,荧光定量PCR实验结果显示,Thil基因在幼叶中的表达量最高,在亚细胞定位中可以发现不同的成员之间在进化过程中分别参与了不同的发育学过程。Thionin-like(Thil)genes possesses activity against pathogenic microorganisms in plant defense system;however,the function of Thil genes is not clear.In order to provide reference for studying its biological function,this study analyzed the sequence composition,gene structure,chromosome distribution,protein conserved domains,phylogenetic tree,gene promoter elements,subcellular localization and expression profile of Thil gene family in barley.Seven barley Thils genes were identified,and they were distributed on chromosome 1,2,4,5 and 6.The gene structure analysis showed that the sequences of the family members were conservative except Thil4.The phylogenetic tree revealed that barley Thil genes were divided into four subfamilies.The results of expression profile analysis demonstrated that the expression of Thil gene varied in different tissues of barley.In general,the expression of Thil gene was very low in the roots and seeds,and the highest in the young leaves.The subcellular localizations of Thil proteins in different members of the family are also different.Thil1 and Thil2 were mainly located in the cell membrane,nucleus and cytoplasm,Thil3 and Thil7 were located in the cell membrane,and Thil6 was located in chloroplast.The Thil gene was associated with plant protection and defense mechanisms.The elements that can stimulate plant protection mechanisms responded most in young leaves.Quantitative RT-PCR(qRT-PCR)results showing the expression of the Thil gene was the highest in young leaves.It can be found that the same members are involved in different developmental processes of plants in the evolutionary process from the subcellular localization experiment.

关 键 词：大麦 类硫素 基因结构 基因表达谱 

分 类 号：S512.3[农业科学—作物学]