黑果枸杞茎叶响应NaCl胁迫合成花色苷的转录组学分析  

Transcriptome Analysis of Lycium ruthenicum Murr.Shoots in Anthocyanin Biosynthesis Response to Salt Stress

作　　者：郭嫒 姜牧炎 哈力马提·巴合太力 刘煜媛 王静 GUO Ai;JIANG Mu-yan;Ha Li-ma-ti·Ba He-tai-li;LIU Yu-yuan;WANG Jing(Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin,National Ethnic Affairs Commission of the People’s Republic of China,College of Biological Science and Engineering,North Minzu University,Yinchuan 750021;Iinnovation Team for Genetic Improvement of Economic Forest,North Minzu University,Yinchuan 750021)

机构地区：[1]北方民族大学生物科学与工程学院国家民委黄河流域农牧交错区生态保护重点实验室,银川750021 [2]北方民族大学经济林遗传改良创新团队,银川750021

出　　处：《生物技术通报》2022年第10期173-183,共11页Biotechnology Bulletin

基　　金：北方民族大学研究生创新项目(YCX21020);北方民族大学中央高校基本科研业务费专项资金资助(2019KJ19);国家自然科学基金项目(32160392);北方民族大学高层次人才项目(2019BJZ02)。

摘　　要：为深入理解黑果枸杞响应盐胁迫合成花色苷的过程,用300 mmol/L NaCl胁迫黑果幼苗,3 d后,收集茎叶,采用液相色谱-串联质谱联用(LC-MS/MS)及转录组测序技术,分别测定其花色苷含量及转录组。通过对花色苷种类、含量及差异表达基因的GO和KEGG分析,挖掘黑果枸杞茎叶响应NaCl胁迫合成花色苷的基因,利用RT-qPCR的方法验证转录组测序结果。结果表明,NaCl处理后,黑果枸杞茎叶中飞燕草素-3-O-芸香糖苷含量升高倍数最高(11.9倍),矮牵牛素-3-O-芸香糖苷含量最高(5.313±0.286)μg/g。共筛选到差异表达基因1416个(P<0.01),其中867个上调,549个下调,功能可归类于催化活性、光合作用、单一有机体代谢过程等GO条目,显著富集于14条KEGG代谢通路。NaCl胁迫后,有7个差异表达基因参与花色苷的合成,显著上调;27个差异表达基因参与植物激素信号转导,其中,15个属ABA信号转导通路;11个MYB和5个bHLH转录因子表达量显著变化。综上所述,ABA信号转导通路、MYB、bHLH转录因子及花色苷合成通路基因的表达变化在黑果枸杞茎叶响应NaCl胁迫合成花色苷的过程中发挥重要作用。In order to analyze the process of Lycium ruthenicum Murr.(L.ruthenicum)shoots in anthocyanin biosynthesis response to salt stress,L.ruthenicum seedlings were treated with 300 mmol/L NaCl,and the shoots were collected at 3 d after treatment,and the anthocyanin content and transcriptome were analyzed by LC-MS/MS and RNA-seq.Combined with the analysis of species and contents of anthocyanin and differential expressed genes(DEGs)in GO and KEGG pathway,DEGs related to anthocyanin biosynthesis induced by salt stress were mined,and the results of transcriptome data were verified by RT-qPCR.The results showed delphinidin-3-O-rutinoside in L.ruthenicum shoots was the highest upregulated anthocyanin(11.9 times),and the content of petunia-3-O-rutinoside was the highest anthocyanin(5.313±0.286)μg/g.Total 1416 DEGs(P<0.01)were screened,including 867 up-regulated and 549 down-regulated DEGs,which were classified into GO terms,such as catalytic activity,photosystem and single-organism metabolic process.DEGs were significantly enriched in 14 KEGG pathways.Meanwhile,there were 7 DEGs involved in anthocyanin biosynthesis and all of them were up-regulated,and27 DEGs were involved in phytohormone signal transduction,in which 15 DEGs were related to ABA signal transduction pathway.The expressions of 11 MYB and 7 bHLH transcription factors were significantly changed.Sum up,the results of this study suggested that DEGs related to ABA pathway,MYB,bHLH transcription factor and anthocyanin biosynthesis play important role in anthocyanin biosynthesis in L.ruthenicum shoot responses to NaCl stress.

关 键 词：黑果枸杞 茎叶 NACL 花色苷 分子调控 

分 类 号：S567.19[农业科学—中草药栽培] Q943.2[农业科学—作物学]