番茄青枯病发生对土壤真菌群落多样性的影响  

Effects of Ralstonia solanacearum Infection on Soil Fungal Community Diversity

作　　者：李婷婷 邓旭辉[1] 李若尘 刘红军 沈宗专[1] 李荣[1] 沈其荣[1] LI Ting-ting;DENG Xu-hui;LI Ruo-chen;LIU Hong-jun;SHEN Zong-zhuan;LI Rong;SHEN Qi-rong(Jiangsu Provincial Key Lab for Organic Solid Waste Utilization,Key Laboratory of Plant Immunity,National Engineering Research Center for Organic-based Fertilizers,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095)

机构地区：[1]江苏省固体有机废弃物资源化高技术研究重点实验室,江苏省有机固体废弃物资源化协同创新中心,作物免疫学重点实验室,南京农业大学资源与环境科学学院,南京210095

出　　处：《生物技术通报》2022年第10期195-203,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(42090065和32002132);江苏省政策引导类计划(国际科技合作/港澳台科技合作)项目(BZ2020026);江苏省自然科学基金(BK20200562);中国博士后科学基金(2020M671520)。

摘　　要：土壤真菌群落对于维持土壤地力及作物健康有着重要的作用,但青枯病发生对土壤真菌群落影响的研究仍相对较少。应用实时荧光定量PCR及MiSeq高通量测序技术,研究了罹患青枯病与未患病番茄植株土体和根际的真菌群落组成。青枯病发生明显改变了番茄土体与根际土壤的真菌群落组成。与未患病根际土壤相比,患病番茄根际土壤的真菌丰度、Chao1值及Shannon值显著降低;潜在土著有益真菌如粘鞭霉属、被孢霉属、顶孢霉属和木霉属的相对丰度显著降低,而有害真菌镰刀菌属的相对丰度显著增加。综上,细菌性青枯病发生影响了土壤真菌群落的组成,其真菌丰度、多样性及土著有益真菌数量降低,而有害真菌数量增加,为阐明土传青枯病发生的微生态机制提供了一定的理论指导。The soil fungal community plays an important role in maintaining soil fertility and plant health,however,the effect of bacterial wilt from Ralstonia solanacearum infection on the soil fungal community still is little.Real-time quantitative PCR and high-throughput sequencing technology of MiSeq were used to study the fungal community composition in the bulk and rhizosphere soils cropped with diseased and non-diseased tomato.The results showed that the occurrence of R.solanacearum infection significantly affected the fungal community composition of bulk and rhizosphere soil.Compared to the non-diseased tomato rhizosphere soil,the fungal abundance,alpha diversity of Chao1 and Shannon in the diseased tomato rhizosphere soil significantly reduced.And the relative abundances of indigenous beneficial fungi such as Gliocladium,Mortierella,Acremonium and Trichoderma significantly reduced whereas the relative abundance of the harmful fungus of Fusarium significantly increased.In summary,the occurrence of R.solanacearum infection altered the composition of the soil fungal community.The fungal abundance,alpha diversity,and the relative abundance of indigenous beneficial fungi depleted while the relative abundance of harmful fungi enriched.The results may provide the elucidation of the microecological mechanism of outbreak of soil-borne bacterial wilt.

关 键 词：青枯病 真菌丰度 真菌多样性 群落组成 

分 类 号：S436.412.15[农业科学—农业昆虫与害虫防治]