一株安全高效的好氧反硝化菌Pseudomonas stutzeri DZ11的生物安全性及脱氮性能研究  

Biosafety and Nitrogen Removal Performance of a Safe and Efficient Aerobic Denitrifying Pseudomonas stutzeri DZ11

作　　者：赵洋 孙慧明 林浩澎 罗娉婷 朱雅婷 陈琼华[1] 舒琥[1] ZHAO Yang;SUN Hui-ming;LIN Hao-peng;LUO Ping-ting;ZHU Ya-ting;CHEN Qiong-hua;SHU Hu(School of Life Science,Guangzhou University,Guangzhou 510006)

机构地区：[1]广州大学生命科学学院,广州510006

出　　处：《生物技术通报》2022年第10期226-234,共9页Biotechnology Bulletin

基　　金：广东省科技计划重点平台项目(2019B030316022);广东省农业农村厅项目(SDYY-2018-10);广东省现代渔业发展专项资金(2017A0003,2017A0007);2021国家级大学生创新创业训练项目。

摘　　要：对中山市民众镇水产养殖场底泥及污水中分离筛选出的好氧反硝化细菌进行研究,旨在为开发养殖废水的脱氮工艺奠定基础。从样品中筛选出高性能菌DZ11,对其进行形态学观察,生理生化测定以及16S rDNA基因序列分析,确定菌株的种属;进行药敏试验和生物安全性检测,确认菌株的安全性;从碳源种类、温度、pH值和碳氮比这4个因素对脱氮效率的影响进行研究,提高菌株的脱氮效率。鉴定结果表明,DZ11为假单胞菌属施氏假单胞菌,并命名为Pseudomonas stutzeri DZ11。经药敏试验和生物安全性检测发现该菌株无明显耐药性并具有较高的生物安全性。单因素优化的结果为:菌株DZ11的最佳碳源为柠檬酸钠,最适温度35.0℃,最佳pH为7,最佳的C/N为10。在分别以氨氮,硝态氮和亚硝态氮为唯一底物时的转化率最高可分别达到84.39%,99.0%和97.65%,说明菌株具有良好的脱氮作用。菌株DZ11具有较高的生物安全性和高效的脱氮性能,在养殖废水的脱氮工艺中具有较大的应用潜力。The aerobic denitrifying bacteria separated and screened from the bottom sludge and sewage of the aquaculture farm in Minzhong town,Zhongshan city,were studied,aiming to lay the foundation for the development of the denitrification process of aquaculture wastewater.The high-performance bacterium DZ11 was selected from the samples,and its morphological observation,physiological and biochemical determination and 16S rDNA gene sequence analysis were performed to determine the species of the strain.Besides,drug susceptibility test and biological safety test were conducted to confirm the safety of strains.Then the influences of carbon source types,temperature,pH value and carbon-nitrogen ratio on the nitrogen removal efficiency were studied to improve the denitrification efficiency of strains’performance.The identification results showed that the strain DZ11 was identified as Pseudomonas stutzeri and named Pseudomonas stutzeri DZ11.Drug susceptibility test and biological safety test demonstrated that the strain had no obvious drug resistance and had high biological safety.The result of single factor optimization is that the optimal carbon source of strain DZ11 was sodium citrate,the optimal temperature was 30.0-35.0℃,the optimal pH was 7,and the optimal C/N was in the range of 10-20.When NH_(4)-N,NO_(3)-N,and NO_(2)-N were the only substrates,the transformation rates were 84.39%,99.0%and 97.65%,respectively,indicating that the strain had a good denitrification effect.In sum,the strain DZ11 has high biological properties and high-efficiency denitrification performance,and has great application potential in the denitrification process of aquaculture wastewater.

关 键 词：好氧反硝化菌 假单胞菌 生物脱氮 生物安全性 

分 类 号：X714[环境科学与工程—环境工程] X172