天祝白牦牛退行期毛囊细胞亚群鉴定以及特征基因生物信息学分析  

Identification of Hair Follicle Cell Subsets and Bioinformatics Analysis of Characteristic Genes in Tianzhu White Yak During Catagen

作　　者：郑青波 叶娜 张哓兰 包鹏甲[2] 王福彬 任稳稳 廖月姣 阎萍[2] 潘和平[1] ZHENG Qing-bo;YE Na;ZHANG Xiao-lan;BAO Peng-jia;WANG Fu-bin;REN Wen-wen;LIAO Yue-jiao;YAN Ping;PAN He-ping(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030;Key Laboratory of Yak Breeding Engineering of Gansu Province,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050)

机构地区：[1]西北民族大学生命科学与工程学院,兰州730030 [2]中国农业科学院兰州畜牧与兽药研究所甘肃省牦牛繁育工程重点实验室,兰州730050

出　　处：《生物技术通报》2022年第10期262-272,共11页Biotechnology Bulletin

基　　金：甘肃省重点研发计划(17YF1NA067);西北民族大学科研创新项目(Yxm2021079)。

摘　　要：从单细胞水平解析天祝白牦牛退行期毛囊发育过程主要细胞类型,旨在对主要细胞类群特异表达基因进行生物功能预测与生物信息学分析,探索退行期毛囊发育调控机制。采用单细胞转录组测序对退行期毛囊进行异质性分析,利用已知标记分子对细胞类群进行筛选鉴定,并对鉴定得到的主要细胞类群进行GO和KEGG分析,同时对毛囊形态发生过程中涉及的关键分子进行免疫组织分析。结果表明,天祝白牦牛退行期涉及IFE-DC细胞、表皮细胞系、黑色素细胞、INFU细胞等细胞类群。其特征基因主要参与表皮发育、上皮细胞分化、超纤维组织发育、组织形态发生以及细胞形态学发生等生物过程。KEGG富集分析发现,特征基因主要富集在黏附连接、细胞周期、RNA转运等与毛囊发育相关的通路中。涉及的不同细胞类型拥有GJA1、FKBP4、KRT1、KRT80、FGFR2等28个共同基因。该研究成功鉴定出天祝白牦牛退行期主要细胞类群,获得了特征基因富集通路,揭示了退行期毛囊发育机制。The main cell types in the hair follicle development process of Tianzhu white yak during the catagen were analyzed at the single cell level,aiming to perform functional prediction and bioinformatics analysis on the characteristic genes of the main cell groups,and to explore the hair follicle development mechanism during catagen.Single cell RNA sequencing was used to analyze the heterogeneity of degenerative hair follicles during catagen,and known marker molecules were used to screen and identify cell groups.GO and KEGG analysis were performed on the main identified cell groups,and immune tissue analysis was performed on the key molecules involved in hair follicle morphogenesis.The results showed that catagen of Tianzhu white yak was involved in IFE-DC cells,epidermal cell lines,melanocytes,INFU and other cell groups.Its characteristic genes were mainly involved in biological processes such as epidermis development,epithelial cell differentiation,super fiber tissue development,tissue morphogenesis and cell morphology.KEGG enrichment analysis discovered that the characteristic genes were mainly enriched in adhesion connection,cell cycle,RNA transport and other pathways related to hair follicle development.The different involved cell types contained 28 common genes such as GJA1,FKBP4,KRT1,KRT80 and FGFR2.This study successfully identified the main cell groups of Tianzhu white yak during catagen,obtained the enrichment pathways of characteristic genes,and revealed the hair follicle development mechanism during catagen.

关 键 词：天祝白牦牛 退行期 毛囊 单细胞转录组测序 生物信息学分析 

分 类 号：S823.85[农业科学—畜牧学]