机械拉伸下人肺上皮细胞增殖及整联蛋白再分布  

Effects of Mechanical Strain on the Proliferation of Pulmonary Epithelial Cells and Reorganization of Integrins

作　　者：张惠静[1] 蔡绍晳[2] 卢晓[2] 王远亮[2] 王红兵[2] 黄岂平[2] 

机构地区：[1]第三军医大学医学检验系,重庆400038 [2]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《生物化学与生物物理学报》2002年第6期748-752,共5页

基　　金：国家自然科学基金资助项目 (No .19732003)~~

摘　　要：应用体外周期性拉伸装置研究机械拉伸对人肺上皮细胞A5 4 9增殖及其膜表面受体———整联蛋白α5、β1再分布的调控作用。结果表明 :在应变为 15 % ,频率为 2 0次 /min、4 0次 /min的拉伸刺激下 ,4 8h后 ,应用流式细胞技术检测细胞的增殖活性指数明显降低 ,A5 4 9细胞的DNA合成受到显著抑制。在 4 0次 /min的拉伸频率下 ,整联蛋白α5、β1的分布发生重组并向基底层转移 ,形成局部粘附连接。研究表明 :整联蛋白α5、β1可能在肺上皮细胞感应机械应力过程中起了重要的作用。Using a cyclic strain unit, the proliferation and reorganization of α 5, β 1 integrins in human pulmonary epithelial A549 cells that underwent mechanical strain were studied. Flow cytometry revealed that cellular proliferative index reduced significantly after cells were subjected to 15% elongation at frequency 20 cycles/min or 40 cycles/min for 48 h. Confocal microscopy revealed that α 5, β 1 integrins in human pulmonary epithelial A549 cells transferred from the apical layer to the basal layer and created adhesion plaques after 24 h exposure to 15% elongation at frequency 40 cycles/min. It is concluded that α 5, β 1 integrins in pulmonary epithelial cells play an important role in transducing mechanical stress into intracelluar signals.

关 键 词：机械拉伸 人肺 上皮细胞增殖 整联蛋白 再分布 粘着斑 

分 类 号：Q66[生物学—生物物理学]