ABCA1、AGT基因和职业紧张交互作用与高血压的关系  

Relationship of hypertension with interactions among ABCA1 gene,AGT gene,and occupational stress

作　　者：付爱玲[1,2] 李欣泽 刘昕 刘继文 FU Ailing;LI Xinze;LIU Xin;LIU Jiwen(School of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830011,China;Medical Record Management Department,The Affiliated Tumor Hospital,Xinjiang Medical University,Urumqi,Xinjiang 830011,China)

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830011 [2]新疆医科大学附属肿瘤医院病案管理科,新疆乌鲁木齐830011

出　　处：《环境与职业医学》2022年第10期1115-1121,共7页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目:(81460489)。

摘　　要：[背景]高血压是基因与环境共同作用导致的疾病。目前关于职业紧张、ATP结合盒转运体A1基因(ABCA1)和血管紧张素原基因(AGT)多态性与高血压关系的研究多为关注单基因或单环境影响。[目的]探讨ABCA1和AGT基因位点多态性和职业紧张交互作用与高血压患病的关系。[方法]将2018年课题组在克拉玛依油田工人中采用随机整群抽样方法调查的1200人中的198例高血压患者作为病例,按照性别、年龄(±3岁)和民族1∶1匹配的原则选择相应的对照组,剔除血样、问卷或DNA纯(浓)度不达标者,最终确定病例组和对照组均为153人。运用调查问卷收集油田工人的一般信息,并通过修订版的职业紧张量表(OSI-R)进行职业紧张程度的评价。运用聚合酶链式反应限制性片段长度多态性技术检测ABCA1 V825I、R219K位点和AGT M235T、T174M位点的基因型。采用广义多因子降维法分析ABCA1和AGT的基因-基因交互作用,以及基因-职业紧张的交互作用与高血压患病率的关系。[结果]病例组和对照组职业紧张程度的分布差异具有统计学意义(P=0.001),病例组的高度职业紧张检出率(65.4%)高于对照组(47.7%)。病例组和对照组的ABCA1 V825I位点、R219K位点,AGT M235T位点基因型和等位基因的分布差异均有统计学意义(P<0.05)。条件logistic回归结果显示,ABCA1 V825I位点VI、II基因型(ORVI=1.682,95%CI:1.099~2.573;ORII=1.708,95%CI:1.045~2.790)、AGT M235T位点TT基因型(OR=1.645,95%CI:1.022~2.647)、高度职业紧张(OR=2.642,95%CI:1.228~5.686)人群高血压患病风险更高(P<0.05);而ABCA1 R219K位点和AGT T174M位点多态性与高血压患病的关联无统计学意义(P>0.05)。ABCA1 V825I、R219K位点与AGT M235T位点之间的基因-基因交互作用与高血压患病有关(训练集和测试集的精确度分别为0.68和0.63,交叉验证一致性系数为10/10,P<0.05),且ABCA1 V825I与AGT M235T位点呈正交互作用;ABCA1 V825I、R219K位点、AGT[Background]Hypertension is influenced by both genes and environment.At present,most studies on the relationship among occupational stress,polymorphisms of ATP binding cassette subfamily A member 1(ABCA1)or angiotensinogen(AGT)genes,and hypertension focus on single gene or single environmental effects.[Objective]To investigate the relationship of potential interactions between ABCA1 and AGT gene polymorphisms and occupational stress with the prevalence of hypertension.[Methods]A total of 198 hypertensive patients were selected as the case group from the 1200 oilfield workers in Karamay Oilfield in 2018 with random cluster sampling method,and the control group was selected as 1∶1 matched subjects for sex,age(±3 years),and ethnicity,after excluding blood samples,questionnaires,or DNA purity(concentration)that did not meet the inclusion criteria.Finally,153 workers in the hypertension case group and 153 workers in the control group were determined.A questionnaire was used to collect general information of the oilfield workers,and the Occupational Stress Inventory Revised Edition(OSI-R)was used to evaluate occupational stress.Polymerase chain reaction-restriction fragment length polymorphism technology was used to detect the genotypes of V825I and R219K loci of ABCA1 as well as M235T and T174M loci of AGT.The gene-gene interaction of ABCA1 and AGT and the relationship between the interaction of gene-occupational stress and the prevalence of hypertension were analyzed by generalized multi-factor dimensionality reduction method.[Results]The difference of reported occupational stress between the hypertension case group and the control group was statistically significant(P=0.001),and the reporting rate of high occupational stress in the case group(65.4%)was higher than that in the control group(47.7%).The genotype and allele distributions of ABCA1 V825I,ABCA1 R219K,and AGT M235T between the hypertension case group and the control group were significantly different(P<0.05).The results of conditional logistic regression

关 键 词：职业紧张 基因多态性 交互作用 高血压 

分 类 号：R135[医药卫生—劳动卫生]