淡竹叶碳量子点的微波法制备及在细胞成像中的应用探究  

Microwave Preparation of Common Lophatherum Herb Carbon Quantum Dots and Application in Cell Imaging

作　　者：胡妙言 刘凯 高诗雨[1,2,3,4] 孙天懿 史继晨 徐长妍 徐丽 HU Miao-yan;LIU Kai;GAO Shi-yu;SUN Tian-yi;SHI Ji-chen;XU Chang-yan;XU Li(College of Materials Science and Engineering,Nanjing Forestry University,Nanjing 210000,China;Jiangsu Co-innovation Centre of Efficient Processing and Utilization of Forest Products,Nanjing Forestry University,Nanjing 210000,China;Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals,Nanjing Forestry University,Nanjing 210000,China;2019 Jiangsu Graduate Workstation:Jingjiang Guolin Wood Industry Co.,Ltd.(Workstation No.2019_099),Jingjiang 214500,China)

机构地区：[1]南京林业大学材料科学与工程学院,江苏南京210000 [2]南京林业大学江苏省林产品高效加工利用联合创新中心,江苏南京210000 [3]南京林业大学绿色生物质燃料与化学品江苏省重点实验室,江苏南京210000 [4]2019年江苏省研究生工作站:靖江国林木业有限公司(工作站编号:2019_099),江苏靖江214500

出　　处：《发光学报》2022年第12期2001-2013,共13页Chinese Journal of Luminescence

基　　金：国家自然科学基金(32071703)资助项目。

摘　　要：利用响应曲面法(RSM)系统研究了微波作用时间(T)、微波功率(W)、淡竹叶与去离子水的料液比(R)对微波法制备淡竹叶氮硅自掺杂碳量子点(N/Si-CQDs)荧光量子产率(QY)的影响。得到了上述工艺参数对QY的影响显著性次序以及对应的QY回归模型与最佳工艺参数,通过验证实验证明优化结果可靠。采用最佳工艺得到的N/Si-CQDs的平均粒径较小且分布均匀,在水中分散性良好,具有激发依赖的发射特性,荧光稳定性较高,对HEK293细胞具有较低细胞毒性,且可被细胞吸收而照亮细胞从而明确区分细胞质和细胞核,说明该碳量子点可用于细胞成像。该研究不仅为淡竹叶的高值化利用提供了一个新思路,而且对提高生物质碳量子点的微波法制备效率、促进其在细胞成像等生物医学领域的应用具有参考价值。In this study,response surface methodology(RSM)was used to study the effects of microwave action time(T),microwave power(W)and the ratio of common lophatherum herb to deionized water(R)on the fluorescence quantum yield(QY)of N/Si-CQDs prepared by microwave method.The significance order of the influence of the above process parameters on QY,and the corresponding QY regression model and the best process parameters are obtained,and the optimization results are proved to be reliable through validation experiments.The average particle size of N/Si-CQDs obtained by the best process is small and evenly distributed,with good dispersion in water,excitation dependent emission characteristics,high fluorescence stability,low cytotoxicity to HEK293 cells.And the N/Si CQDs can be absorbed by cells to illuminate cells,so as to clearly distinguish cytoplasm and nucleus,which shows that the N/Si-CQDs can be used for cell imaging.This study not only provides a new idea for the high-value utilization of bamboo leaves,but also has reference value for improving the efficiency of microwave preparation of biomass carbon quantum dots and promoting their application in biomedical fields such as cell imaging.

关 键 词：淡竹叶 碳量子点 微波法 响应曲面法 细胞成像 

分 类 号：TB383.1[一般工业技术—材料科学与工程]