骨相关间充质干细胞CD166^(+)亚群治疗炎性肠病模型小鼠的研究  

CD166^(+)Subpopulation of Bone-Associated Mesenchymal Stem Cells in the Treatment of Inflammatory Bowel Disease in mouse Models

作　　者：孟炜程 陈要臻 安宁 张婧 陈禹彤 胡兴斌 MENG Wei-cheng;CHEN Yao-zhen;AN Ning(Department of Blood Transfusion,Xijing Hospital,Air Force Military Medical University,Xi'an 710032)

机构地区：[1]空军军医大学第一附属医院输血科,710032

出　　处：《临床输血与检验》2022年第6期693-697,共5页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：国家自然科学基金青年项目(No.31801146);陕西省社发攻关重点研发计划(No.2018SF-199)资助。

摘　　要：目的初步探讨骨相关间充质干细胞CD166^(+)亚群应用于炎症性肠病(IBD)的治疗效果。方法将野生C57BL/6小鼠的股骨和胫骨剔除肌肉再去除骨髓细胞后,胶原酶消化获得骨相关间充质干细胞后,使用间充质干细胞富集试剂盒和CD166阳性磁珠分选试剂盒分选得到CD166^(+)MSCs。使用3%葡聚糖硫酸钠(DSS)诱导IBD小鼠模型,将造模成功的小鼠平均分为CD166^(+)MSCs处理组和对照组,CD166^(+)MSCs处理组小鼠给予静脉输注CD166^(+)MSCs,对照组给予PBS,每日观察小鼠的血便情况和记录体重,输注3 d后检测各组小鼠结肠长度,并做病理切片组织染色分析,观察结肠组织病理改变。结果与对照组相比,CD166^(+)MSCs处理组便血程度明显减轻,结肠长度明显增加,组织病理学切片中的隐窝破坏较少,杯状细胞增多,组织病理学评分较低。结论对于IBD疾病模型移植CD166^(+)MSCs可获得较显著的治疗效果。Objective To investigate the therapeutic effect of the CD166^(+)subpopulation of bone-associated mesenchymal stem cells applied in inflammatory bowel disease(IBD).Methods After removing bone marrow cells from the femur and tibia of C57/BL6 mice,we performed collagenase digestion to obtain bone-associated cells,and sorted CD166^(+)MSCs using an MSC enrichment kit and a CD166-positive magnetic bead sorting kit.A mouse model of IBD was induced using 3%dextran sodium sulfate(DSS),and the successfully molded mice were equally divided into the CD166^(+)MSCs-treated and control groups.Mice in the CD166^(+)MSCs-treated group were given an intravenous infusion of CD166^(+)MSCs,while the control group was given PBS.The mice were observed daily for bloody stool,and body weight was recorded.After three days of infusion,the colon length of mice in each group was examined,and pathological tissue slices were stained and analyzed to observe the histopathological changes in the colon.Results Compared with the control group,the CD166^(+)MSCs-treated group showed significantly less blood in the stool,significantly longer colon length,less destruction of the crypt in pathological tissue slice,more goblet cells,and lower histopathological score.Conclusion Transplantation of CD166^(+)MSCs in the IBD disease model resulted in a better therapeutic effect.

关 键 词：炎症性肠病 骨相关间充质干细胞 CD166 

分 类 号：R392.3[医药卫生—免疫学]