血小板添加剂SSP+与血浆保存血小板的对比研究  

Performances of Platelet Storage in SSP+Additive Solution versus Plasma

作　　者：卿芸 周琼[1] 彭楷[1] 骆展鹏[1] 胡文杰 邹海曼 毛伟[1] QING Yun;ZHOU Qiong;PENG Kai(Institution of Blood Transfusion,Chongqing Blood Center,Chongqing 400052)

机构地区：[1]重庆市血液中心输血研究所,400052

出　　处：《临床输血与检验》2022年第6期698-704,共7页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：重庆市九龙坡区科技计划项目(No.2019-02-019-Y)资助。

摘　　要：目的本研究通过选择经典的血小板添加剂SSP+,建立相对完整且可行的操作方案,比较其与血浆在体外保存混合浓缩血小板的质量。方法随机选取重庆街头采血84人份(400 mL/人份),将同血型的6个新鲜全血用汇集白膜层(BC)方法制备为1个混合浓缩血小板,将其平均分为两份,分别用血小板添加剂SSP+与血浆保存血小板,在保存的第1、5、7天检测其血小板计数、pH值、MPV、血小板代谢指标、低渗性休克反应水平(HSR)、血小板活化指标(CD62p)和凋亡指标(AnnexinⅤ)等。结果用血小板添加剂SSP+保存的血小板浓度、含量、容量、MPV、pO_(2)在保存的7天时间内和血浆组相比差异无统计学意义(P>0.05)。添加剂SSP+组的pH值与血浆组相比差异有统计学意义(P<0.05)。添加剂SSP+组葡萄糖消耗低于血浆组(P<0.05),同时相应乳酸增加量低于血浆组(P<0.05)。随着pCO_(2)的含量降低,CO_(2)的排出增多,添加剂SSP+组中碳酸氢盐的消耗与血浆组相比差异有统计学意义(P<0.05)。添加剂SSP+组HSR高于血浆组,CD62p的表达和AnnexinⅤ均低于血浆组,所有样品在保存的第7天做无菌实验,结果均为阴性。储存至第7天,添加剂SSP+组pH值仍可维持在6.94±0.11,MPV维持在9.89±0.52。结论加入经典添加剂SSP+的血小板在保存的7天内仍能够正常代谢,在血小板代谢、血小板活化、凋亡方面都具有更好的体外指标。Objective We aimed to establish a relatively complete operation procedure for platelet storage using classical SSP+platelet additive solution,and to compare the quality of preserved platelet concentrates in SSP+versus in plasma.Methods Eighty-four blood(400 mL/person)were randomly collected from the general population in Chongqing.A buffy coats product derived from the whole blood donations of 6 same blood type donors were pooled to make a buffy coats platelet concentrate(n=14).Each pool was divided into two groups,suspended in SSP+and plasma,respectively.Platelet count,pH,MPV,platelet metabolism,hypotonic shock response(HSR),CD62p expression and apoptosis indicator(AnnexinⅤ)were measured on Days 1,5 and 7.Results There were no significant differences in platelet concentration,content,volume,MPV and pO_(2) during 7 days of storage between SSP+group and plasma group(P>0.05).However,the pH value in SSP+group was significantly higher than that in plasma group(P<0.05).The glucose consumption in SSP+PAS group and the corresponding increase of lactic acid was significantly lower than that in plasma group(P<0.05).With the decrease of pCO_(2),CO_(2) emission increased.The consumption of bicarbonate in additive SSP+group was significantly lower than that in plasma group(P<0.05).The HSR in SSP+group was higher than that in plasma group.Both the CD62p expression and AnnexinⅤwere lower than that in plasma group.All products were performed the test for sterility on the 7th day of storage,and the results were negative.On the 7th day of storage,the pH and MPV in SSP+group were still remained 6.94±0.11 and 9.89±0.52,respectively.Conclusion Platelets concentrates suspended in the classical SSP+could metabolize normally within 7 days of storage.It has better in vitro parameters in platelet metabolism,platelet activation and apoptosis.

关 键 词：血小板添加剂 SSP+ 血小板保存 

分 类 号：R457[医药卫生—治疗学]