慢性丙型肝炎患者抗核抗体谱表达与HCV-RNA载量及HCV基因型的关联分析  

Correlation Analysis of Antinuclear Antibody Profile Expression with HCV-RNA Load and HCV Genotype in Patients with Chronic Hepatitis C

作　　者：杨艳艳 白顺 YANG Yan-yan;BAI Shun(Department of Laboratory Medicine,the First Affiliated Hospital of University of Science and Technology of China,Hefei 230001)

机构地区：[1]中国科学技术大学附属第一医院检验科,230001

出　　处：《临床输血与检验》2022年第6期767-772,共6页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：国家自然科学基金(No.81901543)资助。

摘　　要：目的分析慢性丙型肝炎患者抗核抗体谱(LIA-ANAs)的表达情况,并探讨LIA-ANAs的表达与HCV-RNA载量以及HCV基因型的关系。方法选取2019年4月~2022年4月就诊于中国科学技术大学附属第一医院的149例慢性丙型肝炎患者为研究对象,同时选取93例体检者作为健康对照组。采用免疫印迹法检测慢性丙型肝炎患者的LIA-ANAs表达水平,采用PCR-荧光探针法检测HCV-RNA载量,PCR-反向点杂交法检测HCV基因型,并对LIA-ANAs与HCV-RNA载量及HCV基因型的关系进行统计分析。结果149例慢性丙型肝炎患者中,HCV-RNA阳性患者56例,阴性患者93例。56例HCV-RNA阳性的慢性丙型肝炎患者中共有25例检出LIA-ANAs,阳性率为44.6%(25/56);93例HCV-RNA阴性的患者中检出21例LIAANAs,阳性率为22.6%(21/93),两组差异有统计学意义(P=0.0048)。将56例HCV-RNA阳性患者分为高拷贝量和低拷贝量两组,两组患者的LIA-ANAs检出阳性率间差异无统计学意义(P=0.5789)。此外,HCV-RNA阳性组抗细胞浆抗体阳性率高于HCV-RNA阴性组,差异有统计学意义(P=0.0204)。HCV-RNA阳性组丙肝患者检出ANA滴度阳性的例数和HCVRNA阴性组相似,差异无统计学意义(P=0.1249)。本研究中患者HCV基因分型主要为1b型,占80.4%(45/56);但HCV各基因型患者血清检出的LIA-ANAs无显著性差异(P=0.244)。结论慢性丙型肝炎患者与健康体检人群相比,血清LIAANAs表达显著升高;活动期的慢性丙型肝炎患者较非活动期患者LIA-ANAs表达显著升高,这提示慢性丙型肝炎患者需要定期监测LIA-ANAs,以防止相关并发症的发生。Objective To analyze the expression of antinuclear antibodies(LIA-ANAs)in patients with chronic hepatitis C and the relationship of LIA-ANAs with HCV-RNA and HCV genotype.Methods A total of 149 patients with chronic hepatitis C and 93 healthy participants from the First Affiliated Hospital of University of Science and Technology of China were recruited in this study from April 2019 to April 2022.The expression levels of LIA-ANAs were detected by western blotting.HCV-RNA and HCV genotype were detected by PCR-fluorescent probe and PCR-reverse dot hybridization,respectively.Results Among the 149 patients,56 were HCV-RNA positive and 93 were negative.LIA-ANAs were detected in 25 of 56 HCV-RNA positive chronic hepatitis C patients,with a positive rate of 44.6%;21 LIA-ANAs were detected in 93 HCV-RNA negative patients,with a positive rate of 22.6%;and the difference between the two groups was statistically significant(P=0.0048).The 56 HCV-RNA positive patients were divided into high and low groups in terms of copy amount.There was no significant difference in the positive rate of LIA-ANAs between the two groups(P=0.5789).Furthermore,the positive rate of cytoplasmic antibody in the HCV-RNA positive group was higher than that in the HCV-RNA negative group,with the difference being statistically significant(P=0.0204).The HCV-RNA positive group was similar in the rate of positive ANA titer to the HCV-RNA negative group(P=0.1249).The main HCV genotype in the patients was type 1b,accounting for 80.4%(45/56);however,no significant difference was observed in the LIA-ANAs detected in the serum of patients with different HCV genotypes(P=0.244).Conclusion Compared with the healthy group,the expression of serum LIA-ANAs in patients with chronic hepatitis C was significantly increased,and the expression of LIA-ANAs in active chronic hepatitis C patients was significantly higher than that in inactive patients.This finding suggests that patients with chronic hepatitis C need regular monitoring of LIA-ANAs to intervene in the occurren

关 键 词：丙型病毒性肝炎 抗核抗体谱 HCV-RNA 基因分型 

分 类 号：R512.63[医药卫生—内科学] R446.6[医药卫生—临床医学]