Proteome-Based Clustering Approaches Reveal Phylogenetic Insights into Amphistegina  

作　　者：Marleen Stuhr Bernhard Blank-Landeshammer Achim Meyer Vera Baumeister Jörg Rahnenführer Albert Sickmann Hildegard Westphal 

机构地区：[1]Leibniz Centre for Tropical Marine Research(ZMT),Bremen,Germany [2]Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V.,Dortmund,Germany [3]Austrian Competence Center for Feed and Food Quality,Safety and Innovation,Wels,Austria [4]Chrestos Concept GmbH&Co KG,Essen,Germany [5]Department of Statistics,TU Dortmund University,Dortmund,Germany [6]Medizinisches Proteom-Center(MPC),Ruhr-Universität Bochum,Bochum,Germany [7]Department of Chemistry,University of Aberdeen,Old Aberdeen,Scotland,UK [8]Department of Geosciences,Bremen University,Bremen,Germany [9]King Abdullah University of Science and Technology(KAUST),Thuwal,Saudi Arabia

出　　处：《Journal of Earth Science》2022年第6期1469-1479,共11页地球科学学刊（英文版）

基　　金：funded by the Leibniz Association(No.SAW-2014-ISAS-2)awarded to Hildegard Westphal,Albert Sickmann and Jorg Rahnenführer。

摘　　要：Foraminifera are highly diverse and have a long evolutionary history.As key bioindicators,their phylogenetic schemes are of great importance for paleogeographic applications,but may be hard to recognize correctly.The phylogenetic relationships within the prominent genus Amphistegina are still uncertain.Molecular studies on Amphistegina have so far only focused on genetic diversity within single species and suggested a cryptic diversity that demands for further investigations.Besides molecular sequencing-based approaches,different mass spectrometry-based proteomics approaches are increasingly used to give insights into the relationship between samples and organisms,especially as these do not require reference databases.To better understand the relationship of amphisteginids and test different proteomics-based approaches we applied de novo peptide sequencing and similarity clustering to several populations of Amphistegina lobifera,A.lessonii and A.gibbosa.We also analyzed the dominant photosymbiont community to study their influence on holobiont proteomes.Our analyses indicate that especially de novo peptide sequencing allows to reconstruct the relationship among foraminiferal holobionts,although the detected separation of A.gibbosa from A.lessonii and A.lobifera may be partly influenced by their different photosymbiont types.The resulting dendrograms reflect the separation in two lineages previously suggested and provide a basis for future studies.

关 键 词：large benthic foraminifera de novo peptide sequencing tandem mass spectra clustering LC-MS/MS runs proteomics symbiont diversity PHYLOGEOGRAPHY Fragilariales 

分 类 号：Q959.11[生物学—动物学]