枸杞多糖影响大鼠背部皮瓣存活的研究  

Effects of lycium barbarum polysaccharide on the survival of dorsal flap in rats

作　　者：张博闻[1,2] 黄永禄[1,2] 马国续[1,2] 巩凡[1,2] 李晓亮 高剑[1,2] 丁一[1,2] 吴建科 张梓炀 赵飞 Zhang Bowen;Huang Yonglu;Ma Guoxu;Gong Fan;Li Xiaoliang;Gao Jian;Ding Yi;Wu Jianke;Zhang Ziyang;Zhao Fei(Department of Hand Surgery,the First Affiliated Hospital of Northwest University for Nationalities(Ningxia Hui Autonomous Region People′s Hospital),Lanzhou 730000,China;Department of Hand and Foot Reconstruction Microsurgery,Autonomous Region People′s Hospital Affiliated to Ningxia Medical University(Ningxia Hui Autonomous Region People′s Hospital),Yinchuan 750002,China)

机构地区：[1]西北民族大学第一附属医院(宁夏回族自治区人民医院),兰州730000 [2]宁夏医科大学附属自治区人民医院(宁夏回族自治区人民医院)手足和重建显微外科,银川750002

出　　处：《中华手外科杂志》2022年第5期425-429,共5页Chinese Journal of Hand Surgery

基　　金：中央高校基本科研业务费专项资金项目(31920190181);宁夏青年科技人才托举工程资助项目(第五批);宁夏重点研发计划项目(2019BEG3044);宁夏自然科学基金(NZ16177);2018年度医院培育振兴科研项目立项(2018013)。

摘　　要：目的探讨枸杞多糖(lycium barbarum polysaccharide,LBP)对大鼠背部任意皮瓣存活状态及术后炎性因子、凋亡因子的影响。方法取84只SD雄性大鼠,随机平均分成4组,在大鼠背部依照样布设计4.5 cm2的任意皮瓣。对照组术后采用生理盐水灌胃(LBP0组),实验组术后用200、400、800 mg/(kg·d)LBP灌胃,分别为LBP200、LBP400、LBP800组,持续时间为2周。记录各组大鼠皮瓣术后的一般情况,包括炎性渗出、水肿、皮瓣张力、皮温及坏死。各组分别于术后0.5、1、3、5、7、10及14 d随机选取3只大鼠测算皮瓣存活面积。切取皮瓣组织样本,采用实时荧光定量PCR(RT-PCR)检测皮瓣组织中TNF-α、Caspase-3因子的mRNA表达。结果大鼠背部任意皮瓣造模后3 d,各组皮瓣创面均可见不同程度渗血及肿胀,部分皮瓣逐步出现暗红至青紫,术后7 d皮瓣坏死逐渐显现,术后10 d坏死边界渐清晰,术后2周坏死区域结痂翘起。各组大鼠术后1周内,皮瓣存活面积差异无统计学意义(P>0.05)。术后3 d,LBP800与LBP0组相比,TNF-α因子mRNA表达差异有统计学意义(P<0.05),且在术后10 d差异更明显(P<0.01)。术后5 d,LBP400与LBP0组相比,TNF-α因子mRNA表达量也存在差异(P<0.05);LBP800与LBP0组相比,Caspase-3因子mRNA表达量存在差异(P<0.05),且术后14 d两组差异更明显(P<0.01)。术后7 d,LBP400与LBP0组相比,Caspase-3因子mRNA表达量存在明显差异(P<0.05)。结论适宜浓度的LBP干预可显著降低皮瓣炎性反应因子及凋亡因子的mRNA表达,有助于降低皮瓣组织坏死的风险,改善皮瓣存活微环境。Objective To investigate the effects of lycium barbarum polysaccharide(LBP)on the survival state of rat dorsal random flap and postoperative inflammatory factors and apoptotic factors.Methods Eighty-four male SD rats were randomly divided into 4 groups,and 4.5 cm2 random flaps were designed on the back of the rats according to the sample cloth.The control group was perfused with normal saline(group LBP0)after operation,and the experimental group was perfused with 200,400 and 800 mg/(kg·d)LBP after operation,which were group LBP200,LBP400 and LBP800 respectively,lasting for 2 weeks.The general conditions of rats in each group after flap operation were recorded,including inflammatory exudation,edema,flap tension,skin temperature and necrosis.Three rats in each group were randomly selected at 0.5,1,3,5,7,10 and 14 days after operation to measure the survival area of the flap.The flap tissue samples were cut and the mRNA expression of TNF-αand Caspase-3 factor in the flap tissue was detected by real-time fluorescent quantitative PCR(RT-PCR).Results Three days after the modeling of rat dorsal random flap,bleeding and swelling in varying degrees were observed in the wound of the flap in each group,and some flaps gradually appeared dark red to cyan.The necrosis of the flap gradually appeared on the 7th day after the operation,and the necrosis boundary became clear on the 10th day after the operation.The scabs were formed in the necrotic area 2 weeks after the operation.Within one week after operation,there was no significant difference in the survival area of the flap in each group(P>0.05).Three days after operation,the difference of mRNA expression of TNF-αbetween group LBP800 and LBP0 was significant(P<0.05),and the difference was more obvious 10 days after operation(P<0.01).Five days after operation,the difference of mRNA expression of TNF-αbetween group LBP400 and LBP0 was significant(P<0.05),and the difference of mRNA expression of Caspase-3 factor between group LBP800 and LBP0 was significant(P<0.05),with more

关 键 词：动物实验 大鼠 外科皮瓣 枸杞多糖 炎性因子 凋亡因子 

分 类 号：R285.5[医药卫生—中药学]